BCMA / Luciferase - CHO Recombinant Cell Line

Catalog #: 79724
Size: 2 vials
B-Cell Maturation Antigen (BCMA), also known as CD269, is a cell surface receptor of the TNF receptor superfamily that recognizes B-Cell Activating Factor (BAFF). BCMA is preferentially expressed on mature B-lymphocytes and Multiple Myeloma (MM) cells. BCMA is a highly attractive target antigen for immunotherapy not only because of its restricted expression in non-malignant tissue, but also due to its almost universal expression on MM cells. Pre-clinical studies using CAR (Chimeric Antigen Receptor) T-cells targeting BCMA have demonstrated anti-MM activity, and in 2017, the FDA granted BCMA CAR T-Cell immunotherapy the breakthrough designation in treating Multiple Myeloma.
Recombinant CHO-K1 cells constitutively expressing both the human BCMA protein (B-Cell Maturation Antigen or CD269, GenBank accession #NM_001192) and the firefly luciferase reporter. Surface expression of BCMA was confirmed by flow cytometry.
Materials Required But Not Provided:

Thaw Medium 3 (BPS Bioscience, #60184)
Growth Medium 3A (BPS Bioscience, #60188)
• 96-well tissue culture-treated white clear-bottom assay plate
ONE-Step luciferase assay system (BPS Bioscience, #60690) or other luciferase
reagent for measuring firefly luciferase activity
• Luminometer

TNFRSF17, BCM, BCMA, CD269, TNFRSF13A, tumor necrosis factor receptor superfamily member 17
Supplied As:
Each vial contains ~ 2 x 10^6 cells in 1 ml of 10% DMSO in FBS.
Host Cell line:
CHO K1. Chinese Hamster Ovary.
Mycoplasma Testing:
This cell line has been screened using the MycoAlertâ„¢ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control.
Culture Conditions:

Thaw medium 3 (BPS Bioscience, #60186): Ham’s F-12 medium (Hyclone # SH30526.01) supplemented with 10% FBS (Thermo Fisher, Cat. #26140079), 1% Penicillin/Streptomycin (Hyclone SV30010.01).

Growth Medium 3A (BPS Bioscience #60188): Thaw medium 3 (BPS Bioscience, #60186) plus 1 mg/ml of Geneticin (Thermo Fisher, #11811031) and 500 μg/ml of Hygromycin B (Thermo Fisher, #10687010) to ensure recombinant expression.

Instructions for use:
See data sheet for detailed culturing and assay protocol.
Storage / Stability:
Store in liquid nitrogen immediately upon receipt.
Use as BCMA-expressing target cells in co-culture assays with BCMA CAR T-cells to screen for BCMA-specific cell killing

License Disclosure Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Avoid multiple freeze/thaw cycles.
Scientific Category:
Product Type:
Cell Line
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