BCMA / NF-κB – Reporter HEK293 Recombinant Cell Line

Catalog #

79755

$11,375 *

Size: 2 vials

Qty

Bulk/Custom

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Description

The BCMA / NF-κB - HEK293 recombinant cell line has been transfected with full-length human BCMA cDNA (Genbank #NM_001192) under control of a CMV promoter for high constitutive expression. The NF-κB–luciferase reporter is also stably integrated into the genome. The firefly luciferase gene is controlled by four copies of the NF-κB Response Element upstream of a minimal promoter. Upon ligand binding, active BCMA will initiate the NF-κB signaling pathway, leading to expression of the NF-κB-controlled luciferase reporter.

Interested in screening and profiling inhibitors, blocking antibodies, or activators of BCMA signaling without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

tumor necrosis factor receptor superfamily member 17, B-Cell Maturation Antigen, TNFRSF17, CD269, nfkb, nf-kb

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Product Data Gallery

Dose response of NF-κB reporter activity to BAFF in the presence of 0.5% FBS

Dose response of NF-κB reporter activity to APRIL in the presence of 0.5% FBS

Product Info

Storage and Usage

Citations

Supplied As

Two vials containing ~ 2 x 106 cells in 1 ml of 10% DMSO in FBS

Materials Required But Not Supplied

• Thaw Medium 1 (BPS Bioscience, #60187)
• Growth Medium 1A (BPS Bioscience #79528)
• Assay Medium 7B (BPS Bioscience, #79718):
o Opti-MEM I (Life Technologies #31985-062) supplemented with 0.5% FBS
(Thermo Fisher, #26140079), 1% non-essential amino acids (Hyclone,
#SH30238.01), 1 mM Na pyruvate (Hyclone, #SH30239.01), 1%
Penicillin/Streptomycin (Hyclone, #SV30010.01).
• Recombinant human BAFF (BPS Bioscience, #100194)
• Recombinant human APRIL (BPS Bioscience, #100254)
• 96-well tissue culture plate or 96-well tissue culture-treated white clear-bottom assay
plate
• ONE-Step™ Luciferase Assay System (BPS Bioscience, #60690)
• Luminometer

UniProt #

Q02223

Mycoplasma Testing

This cell line has been screened using the Venor™ GeM Mycoplasma Detection Kit, PCR Based (Sigma, #MP0025) to confirm the absence of Mycoplasma contamination.

Background

B-Cell Maturation Antigen (BCMA), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17) or CD269, is a type I membrane protein encoded by the TNFRSF17 gene. TNFRSF17 is a cell surface receptor of the TNF receptor superfamily, that recognizes its ligands including BAFF (B-cell activating factor) and APRIL (a proliferation inducing ligand). BCMA is preferentially expressed in mature B lymphocytes and also on Multiple Myeloma (MM) cells. In vitro, the activation of BCMA by its ligand promotes the differentiation and proliferation of B cells. A vast array of intracellular activity is involved in BCMA-induced signal transduction, including activation of the NF-κB signaling pathway. The bioactivity of BAFF and APRIL as soluble homotrimers distinguishes them from other TNFSF ligands such as TRAIL, FasL, and CD40L, which are only active as membrane-bound molecules.

References

Hahne, M. et al. (1998) J. Exp. Med. 188:1185.
Yu, G. et al. (2000) Nat. Immunol. 1:252.
Yan, M. et al. (2000) Nat. Immunol. 1:37.
Gravestein, L.A. and Borst, J. (1998) Sem. Immunol. 10:423.

Storage/Stability

Store in liquid nitrogen immediately upon receipt. Do not store long-term at -80°C or on dry ice.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 1 (BPS Bioscience, #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin

Growth Medium 1A (BPS Bioscience, #79528): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin, 400 μg/ml of Geneticin and 100 μg/ml Hygromycin B

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid multiple freeze/thaw cycles.

Scientific Category

CAR T-Cell Therapy

Regulatory Status

Research Use Only

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BCMA / NF-κB – Reporter HEK293 Recombinant Cell Line

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