BCMA:APRIL[Biotinylated] Inhibitor Screening Assay Kit
The BCMA:APRIL[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of BCMA:APRIL. The key to this kit is the high sensitivity of detection of biotin-labeled APRIL by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, BCMA is coated on a 96-well plate. Next, biotinylated APRIL is incubated with BCMA on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.
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PBS (Phosphate buffered saline)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Catalog # | Component | Amount | Storage | |
79465 | BCMA, Fc-Fusion, Avi-Tag HiP™ | 10 µg | -80°C | Avoid multiple freeze/ thaw cycles! |
100262 | APRIL, His-Avi-Tag, Biotin-Labeled | 5 µg | -80°C | |
79311 | 3x Immuno Buffer 1 | 50 ml | -20°C | |
79728 | Blocking Buffer | 50 ml | +4°C | |
79742 | Streptavidin-HRP | 15 µl | -20°C | |
79670 | ELISA ECL Substrate A | 6 ml | +4°C | |
ELISA ECL Substrate B | 6 ml | +4°C | ||
79699 | 96-well white microplate | 1 | +4°C |
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