Anti-BCMA CAR-T Cells

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78660

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Description

The anti-BCMA CAR-T cells are produced by high-titer lentiviral transduction of human primary CD4+CD8+ T cells using the anti-BCMA CAR Lentivirus (BPS Bioscience #78655). These ready-to-use CAR-T cells express an anti-BCMA CAR consisting of the ScFv (Single chain fragment variable) of anti-BCMA (clone C11D5.3) linked to a 2^nd generation CAR (Chimeric Antigen Receptor) containing CD8 hinge and transmembrane domains, and the 4-1BB and CD3ζ signaling domains (Figure 1).

These CAR-T cells have been validated using flow cytometry (to determine the CAR expression) and co-culture cytotoxicity assays.

Figure 1: Construct diagram showing components of the anti-BCMA CAR expressed in anti-BCMA CAR-T cells.

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Product Data Gallery

Expression of anti-BCMA CAR in anti-BCMA CAR-T cells

Luciferase-based cytotoxicity assay using BCMA-Luciferase CHO cells as the target cells

Luciferase-based cytotoxicity assay using Firefly Luciferase-RPMI8226 Recombinant Cell Line as the target cells

Product Info

Storage and Usage

Citations

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of CryoStor® CS10 (Stemcell Technologies)

Materials Required But Not Supplied

Name	Ordering Information
Human Interleukin-2	BPS Bioscience #90184
Human CD3/CD28/CD2 T Cell Activator	Stemcell Technologies #10970
BCMA/Firefly Luciferase CHO Cell Line	BPS Bioscience #79724
Firefly Luciferase CHO Cell Line	BPS Bioscience #79725
Firefly Luciferase RPMI8226 Cell Line	BPS Bioscience #79834
Untransduced T Cells (Negative Control for CAR-T cells)	BPS Bioscience #78170
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
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Recommended anti-BCMA CAR-T Cell Medium: TCellM™ (BPS Bioscience #78753) supplemented with 10 ng/ml Interleukin-2 (BPS Bioscience #90184).

Mycoplasma Testing

The cells have been screened to confirm the absence of Mycoplasma species.

Background

B-cell maturation antigen (BCMA), also known as CD269 or tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a cell surface receptor of the TNF receptor superfamily that recognizes B-cell activating factor (BAFF). BCMA is preferentially expressed in mature B lymphocytes and on Multiple Myeloma (MM) cells. BCMA is a highly attractive target antigen for immunotherapy because of its restricted expression in nonmalignant tissue but almost universal expression on MM cells. So far FDA has approved two BCMA CAR-T therapies for the treatment of multiple myeloma.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Applications

Positive control for anti-BCMA CAR-T cells
Screen modulators of anti-BCMA CAR-T cytotoxicity
Design and optimize co-culture cytotoxicity assay

Shipping Temperature

-80°C

Notes

Biosafety
The anti-BCMA CAR-T cells are produced with the third generation SIN (self-inactivation) lentivector which ensures self-inactivation of the lentiviral construct after transduction and integration into the genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal and are not present in the lentivirus particle.

Troubleshooting Guide
Visit Cell Line FAQs for more information.
For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Donors have been screened and determined negative for:

Hepatitis B (anti-HBc EIA, HBsAg EIA)
Hepatitis C (anti-HCV EIA)
Human Immunodeficiency Virus (HIV-1/HIV-2 plus O)
Human T-Lymphotropic Virus (HTLV-I/II)
HIV-1/HCV/HBV
West Nile Virus
Trypanasoma cruzi

Note: Testing cannot guarantee that any sample is completely virus-free. These cells should be treated as potentially infectious and appropriate biological safety level 2 precautions should be used.

Scientific Category

CAR-T

Regulatory Status

Research Use Only

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Anti-BCMA CAR-T Cells

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