Firefly Luciferase – RPMI 8226 Recombinant Cell Line

Catalog #

79834

$2,045 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Recombinant RPMI 8226 cells stably expressing firefly (Photinus pyralis) luciferase. These cells naturally express human B-Cell Maturation Antigen (BCMA) and represent a relevant cell line-derived xenograft (CDX) model.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

Luciferase activity of Firefly Luciferase RPMI 8226 recombinant cells.

Product Info

Storage and Usage

Citations

Host Cell Line

RPMI 8226, human B cells isolated from a plasmacytoma/myeloma patient, suspension.

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of cell freezing medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Name	Ordering Information
Thaw Medium 10	BPS Bioscience #79704
Growth Medium 10A	BPS Bioscience #79835

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

RPMI 8226 cells are human B cells isolated from a plasmacytoma/myeloma patient. RPMI 8226 cells constitutively express BCMA and offer a physiologically relevant platform to evaluate cancer-directed immunotherapies, such as Chimeric Antigen Receptor (CAR) T cells. The Firefly Luciferase RPMI 8226 Cell Line makes an excellent target to measure specific killing by CAR-T or NK cells targeting BCMA or other targets of interest.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 10 (BPS Bioscience, #79704): RPMI 1640 medium supplemented with 10% FBS, 1 mM Sodium pyruvate, 1% Non-essential amino acids, 1% Penicillin/streptomycin.

Growth Medium 10A (BPS Bioscience, #79835): RPMI 1640 medium supplemented with 10% FBS, 1 mM Sodium pyruvate, 1% Non-essential amino acids, 1% Penicillin/streptomycin with 400 μg/ml of Geneticin to ensure recombinant expression.

Applications

Use as a target cell in co-culture cytotoxicity assays.
Perform bioluminescence imaging of tumor growth in vivo.
Assess the effect of therapeutic compounds on cell proliferation in vivo and in vitro.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only