ISRE Luciferase Reporter Lentivirus (JAK/STAT Signaling Pathway)

Catalog #

79824

$820 *

Size: 500 µl x 2

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

The JAK/STAT pathway is activated by various cytokines and growth factors and plays a critical role in cell growth, hematopoiesis, and immune response. In mammals, there are four JAKs (JAK1, JAK2, JAK3 and TYK2) and seven STAT proteins. IFNα is a Type I interferon. Binding of IFNα to its receptor leads to the activation of JAK1 and TYK2, which in turn phosphorylate and activate STAT1 and STAT2. The phosphorylated STAT1 and 2 form a heterodimer and bind to IRF9/p48, forming a protein complex ISGF3. This complex translocates to the nucleus and binds to the ISRE (Interferon Stimulated Response Element) in the promoter region, thereby promoting transcription of interferon-inducible genes.

The ISRE Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by multimerized ISRE response element located upstream of the minimal TATA promoter (Figure 1). After transduction, the activity of Type I interferon-induced JAK/STAT signaling pathway in the target cells can be monitored by measuring the luciferase activity.

Figure 1. Schematic of the lenti-vector used to generate the ISRE luciferase reporter
lentivirus

This product has been cited 2 times.

●

Synonyms

JAK reporter, STAT reporter, ISRE reporter, IFN, lentivirus reporter,

●

Product Data Gallery

Product Info

Storage and Usage

Citations2

Supplied As

Two vials (500 μl x 2) of ISRE luciferase reporter lentivirus at a titer 5 x 10⁶ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Materials Required But Not Supplied

• Human Interferon Alpha A (IFNα) (R&D Systems # 11100-1)
• STING Ligands: 2,3 cGAMP (Invivogen # tlrl-nacga23), 3,3 cGAMP (Invivogen #tlrlnacga)
• THP-1 cell (ATCC#TIB-202)
• THP-1 growth medium or use Thaw Medium 8 (BPS Bioscience #79652)
• Assay medium: RPMI 1640 medium (Life Technologies #A10491-01)
• Polybrene (Millipore, #TR-1003-G)
• 96-well tissue culture treated white clear-bottom assay plate (Corning, #3610)
• One-Step luciferase assay system (BPS Bioscience, #60690)
• Luminometer

Formulation

The lentiviruses were produced from HEK293T cells in the medium containing 90% DMEM + 10% FBS.

References

1. Hebenstreit, D., et al. (2005). JAK/STAT-dependent gene regulation by cytokines. Drug News Perspect 18 (4): 243–249.

Storage/Stability

Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Notes

Biosafety

The lentiviruses are produced with the third generation SIN (self-inactivation) lentivector which
ensures self-inactivation of the lentiviral construct after transduction and integration into the
genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the
transduced cells, as they are expressed from packaging plasmids lacking the packing signal.
Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a
Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional
regulations and using all appropriate safety precautions

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

ISRE Luciferase Reporter Lentivirus (JAK/STAT Signaling Pathway)

PRODUCTS

SERVICES

RESOURCES

COMPANY