ISRE Reporter Kit (JAK/STAT Signaling Pathway)

Catalog #: 60613
Size: 500 reactions
The JAK (Janus kinase) /STAT (Signal Transducer and Activator of Transcription) pathway is activated by various cytokines and growth factors and plays a critical role in cell growth, hematopoiesis, and immune response. In mammals, there are four JAKs (JAK1, JAK2, JAK3 and TYK2) and seven STAT proteins. Interferon alpha (IFNa) is a Type I interferon. Binding of IFNa to its receptor leads to the activation of JAK1 and TYK2, which in turn phosphorylate and activate STAT1 and STAT2. The phosphorylated STAT1 and 2 form a heterodimer and bind to IRF9/p48, forming a protein complex ISGF3. This complex translocates to the nucleus and binds to the ISRE (Interferon Stimulated Response Element) in the promoter region thereby promoting transcription of interferon-inducible genes.
The ISRE Reporter kit is designed for monitoring the activity of Type I interferon-induced JAK/STAT signaling pathway in the cultured cells. The kit contains transfection-ready ISRE luciferase reporter vector, which is a JAK/STAT pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized ISRE responsive element located upstream of a minimal promoter. The ISRE reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The noninducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity.
JAK reporter, STAT reporter, ISRE reporter, IFN, Cell based assay kit, cell-based assay, reporter assay kit
Supplied As:
Reporter: ISRE luciferase reporter vector + constitutively-expressing Renilla luciferase vector
Negative Control Reporter: Non-inducible luciferase vector + constitutively-expressing Renilla luciferase vector

Both the Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each)

assay kit
Instructions for use:
See data sheet insert for detailed transfection and assay protocols.
Storage / Stability:

Stable at least 12 months from date of receipt, when stored as directed (-20°C)

• Monitor IFNa-induced JAK/STAT signaling pathway activity.
• Screen for activators or inhibitors of the JAK/STAT signaling pathway.
• Study effects of RNAi or gene overexpression on the activity of the JAK/STAT pathway.

The vectors are suitable for transient transfection. They are NOT suitable for transformation and amplification in bacteria.

Avoid freeze/thaw cycles
Product Type:
Reporter Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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