STAT3 Luciferase Reporter Lentivirus

Catalog #

79744

$860 *

Size: 500 µl x 2

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The STAT3 Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene under the control of STAT3-responsive element located upstream of the minimal TATA promoter (Figure 1). After transduction, activation of the STAT3 signaling pathway in the target cells can be monitored by measuring the luciferase activity.

Figure 1. Schematic of the lenti-vector used to generate the STAT3 luciferase reporter lentivirus.

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Synonyms

Acute-Phase Response Factor, APRF, ADMIO1, HIES, STAT-3

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Product Info

Storage and Usage

Citations

Supplied As

Two vials (500 μl x 2) of STAT3 luciferase reporter lentivirus at a titer ≥5 x 10^6 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Materials Required But Not Supplied

• IL-6 (BPS Bioscience, #90196-B)
• HEK293 growth medium or use Thaw Medium 9 (BPS Bioscience, #79665): MEM with
10% FBS, 0.1 mM nonessential amino acids, 1 mM sodium pyruvate
• Polybrene (Millipore, #TR-1003-G)
• 96-well tissue culture treated white clear-bottom assay plate (Corning, #3610)
• One-Step luciferase assay system (BPS Bioscience, #60690)
• Luminometer

Formulation

The lentiviruses were produced from HEK293T cells and are provided in medium containing 90% DMEM + 10% FBS.

References

1. Pessara, U., Koch, N. (1990) Tumor necrosis factor alpha regulates expression of the major histocompatibility complex class II-associated invariant chain by binding of an NF-κB-like factor to a promoter element. Mol Cell Biol. 10(8):4146-4154.

2. Baeuerle, P.A. (1998) Pro-inflammatory signaling: last pieces in the NF-κB puzzle? Curr Biol. 8(1):R19-R22.

Storage/Stability

Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.

Instructions for Use

See assay protocol for detailed instructions,

Shipping Temperature

-80°C

Notes

Biosafety:
The lentiviruses are produced with the second generation SIN (self-inactivation) lentivector which ensures self-inactivation of the lentiviral construct after transduction and integration into the genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal.
Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

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STAT3 Luciferase Reporter Lentivirus

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