STAT3 Reporter Kit (STAT3 Signaling Pathway)

Catalog #

79730

$640 *

Size: 500 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The STAT3 Reporter kit is designed for monitoring the activity of the STAT3 signaling pathway in cultured cells. The kit contains transfection-ready STAT3 luciferase reporter vector. This reporter contains a firefly luciferase gene under the control of STAT3-responsive element located upstream of a minimal promoter. The STAT3 reporter is premixed with constitutively expressing Renilla luciferase vector, which serves as an internal control for transfection efficiency.

The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity.

For assays in more difficult to transfect cell types, we suggest using our lentiviral products. The corresponding lentiviral product for this kit is BPS Bioscience #79744.

This product has been cited 5 times.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Acute-Phase Response Factor, APRF, ADMIO1, HIES, STAT-3

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Product Data Gallery

Product Info

Storage and Usage

Citations5

Materials Required But Not Supplied

• Mammalian cell line and appropriate cell culture medium
• 96-well tissue culture plate or 96-well tissue culture treated white clear-bottom
assay plate (Corning #3610)
• Transfection reagent for mammalian cell line [We use Lipofectamine™ 2000
(Invitrogen #11668027). However, other transfection reagents work equally well.
• Opti-MEM I Reduced Serum Medium (Invitrogen #31985-062)
• TWO-Step Luciferase (Firefly & Renilla) Assay System (BPS Bioscience #60683): This
system assays cells directly in growth medium. It can be used with any
luminometer. Automated injectors are not required.
• Luminometer

Format

Component

Specification

Amount

Storage

Reporter

(Component A)

STAT3 luciferase reporter vector + constitutively expressing Renilla luciferase vector

500 ml

(60 ng DNA/ ml)

-20°C

Negative Control Reporter

(Component B)

Non-inducible luciferase vector + constitutively expressing Renilla luciferase vector

500 ml

(60 ng DNA/ ml)

-20°C

UniProt #

P40763

Background

STAT3 (Signal Transducer and Activator of Transcription 3) is a transcription factor which is activated through phosphorylation at Tyrosine 705 in response to various cytokines and growth factors. The phosphorylated STAT3 forms homodimers or heterodimers with STAT1 and the dimers translocated to the nucleus where DNA binding/promoter induction occurs. STAT3 plays an important role in cellular processes such as cell cycle progression and apoptosis.

References

1. Tian S., et al., Blood. 1994; 84(6):1760-1764.

2. Zhong, Z., et al., Science. 1994; 264(5155):95-98.

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STAT3 Reporter Kit (STAT3 Signaling Pathway)

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