STAT3 eGFP Reporter Lentivirus

Catalog #

78197

$795 *

Size: 500 µl x 2

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The STAT3 eGFP Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain an eGFP gene under the control of a STAT3-responsive element located upstream of the minimal TATA promoter (Figure 1). After transduction, activation of the STAT3 signaling pathway in the target cells can be monitored by examining eGFP expression.

Figure 1. Schematic of the lenti-vector used to generate the STAT3 eGFP reporter lentivirus

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Synonyms

Acute-Phase Response Factor, APRF, ADMIO1, HIES, signal transducer and activator of transcription 3, STAT-3

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Product Info

Storage and Usage

Citations

Species

Replication Incompetent HIV

Supplied As

Two vials (500 µl x 2) of STAT3 eGFP lentivirus at a titer ≥ 10⁷ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Materials Required But Not Supplied

Name	Ordering Information
Human IL-2	BPS Bioscience, #90196-B
Thaw Medium 9	BPS Bioscience, #79665
Polybrene	Millipore, #TR-1003-G
96-well tissue culture treated white clear-bottom assay plate	Corning, #3610
ONE-Step™ luciferase assay system	BPS Bioscience, #60690
Luminometer

Formulation

The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS.

Storage/Stability

Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C.

Applications

Screen for activators or inhibitors of the STAT3 signaling pathway in transduced target cells
Generation of STAT3 eGFP reporter stable cell line

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.

Biosafety

The lentiviruses are produced with the third-generation SIN (self-inactivation) lentivector which ensures self-inactivation of the lentiviral construct after transduction and integration into the genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

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STAT3 eGFP Reporter Lentivirus

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