STAT3 Reporter (eGFP)-HEK293 Cell Line

Catalog #

78319

$3,390 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The STAT3 Reporter (eGFP)-HEK293 cell line is designed to monitor the STAT3 signal transduction pathway. It contains an eGFP gene driven by STAT3 response elements located upstream of the minimal TATA promoter. After activation by cytokines and growth factors, endogenous STAT3 binds to the DNA response elements, inducing transcription of the eGFP reporter gene.

Interested in screening and profiling inhibitors, blocking antibodies, or activators of STAT3-mediated signaling pathways without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Acute-Phase Response Factor, DNA-Binding Protein APRF, ADMIO, Signal Transducer And Activator Of Transcription 3, STAT-3

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Product Data Gallery

Inhibition of IL-6-induced Reporter Activity by Anti-IL-6R Antibody in STAT3 (eGFP) Reporter HEK293 Cells.

Product Info

Storage and Usage

Citations

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of 10% DMSO

Materials Required But Not Supplied

Materials Required for Cell Culture

Name	Ordering Information
Thaw Medium 1	BPS Bioscience #60187
Growth Medium 1N	BPS Bioscience #79801

Materials Required for Cellular Assay

Name	Ordering Information
Human IL-6	R&D Systems #206-IL
Anti-IL-6R antibody	R&D Systems #MAB227
96-well tissue culture treated white clear-bottom assay plate	Corning #3610

Formulation

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin

Growth Medium 1N (BPS Bioscience #79801): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin plus 0.5 µg/ml of Puromycin

Assay Medium: Thaw Medium 1 (BPS Bioscience #60187)

UniProt #

P40763

Mycoplasma Testing

The cell line has been screened using the MycoAlert™ Mycoplasma Detection kit (Lonza, #LT07-218) to confirm the absence of Mycoplasma species.

Storage/Stability

Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt.

Instructions for Use

See assay protocol for detailed instructions

Applications

• Monitor the STAT3 signaling pathway activity
• Screen for activators or inhibitors of the STAT3 signaling pathway

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles. Do not use a -80°C freezer for long term storage.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only