STAT3 Luciferase Reporter HEK293 Cell Line

Catalog #

79800-P

$3,730 *

Size: 2 vials

Qty

Bulk/Custom

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Description

The STAT3 Luciferase Reporter HEK293 Cell Line is a HEK293 cell lines expressing firefly luciferase driven by STAT3 response elements located upstream of a minimal TATA promoter. After activation by cytokines or growth factors, endogenous STAT3 binds to the response elements, inducing transcription of the luciferase reporter.

This cell line has been validated to respond to human interleukin-6 (IL-6) and human IL-27. Functional validation experiments showed that IL-6-induced luciferase activity was decreased by the JAK inhibitor CP 690,550 and by an anti-IL-6 Receptor neutralizing antibody.

Figure 1: Illustration of the mechanism behind reporter expression in the STAT3 Luciferase Reporter HEK293 Cell Line.
Created with BioRender.com

Interested in screening and profiling inhibitors, blocking antibodies, or activators of STAT3-mediated signaling without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

This product has been cited 1 time.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Acute-Phase Response Factor, DNA-Binding Protein APRF, ADMIO, Signal Transducer And Activator Of Transcription 3, STAT-3

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Product Data Gallery

Dose response curve of STAT3 Luciferase Reporter HEK293 Cell Line to hIL-6

Dose response curve of STAT3 Luciferase Reporter HEK293 Cell Line to hIL-27

Inhibition of hIL-6-induced reporter activity by JAK Inhibitor CP 690,550 in STAT3 Luciferase Reporter HEK293 Cell Line

Inhibition of hIL-6-induced reporter activity by anti-IL-6R antibody in STAT3 Luciferase Reporter HEK293 Cell Line

Product Info

Storage and Usage

Citations1

Supplied As

Each vial contains >1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Materials Required for Cell Culture

Name	Ordering Information
Thaw Medium 1	BPS Bioscience #60187
Growth Medium 1N	BPS Bioscience #79801

Materials Required for Cellular Assay

Name	Ordering Information
Human IL-6	R&D Systems #206-IL
JAK inhibitor CP 690,550	Caymaan #11598
Anti-IL-6R anitbody	R&D Systems #MAB227
Assay Medium: Thaw Medium 1	BPS Bioscience #60187
Growth Medium 1N	BPS Bioscience #79801
96-well tissue culture treated white clear-bottom assay plate	Corning #3610
ONE-Step™ luciferase assay system	BPS Bioscience #60690
Luminometer

UniProt #

P40763

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

STAT3 (Signal Transducer and Activator of Transcription 3) is a transcription factor expressed in diverse cell types. STAT3 is phosphorylated by JAK (Janus Kinases) and gets translocated to the nucleus. While it was initially described as an acute phase response factor in the context of IL-6 signaling, STAT3 has since been identified as a downstream effector of many cytokines. STAT3-mediated IL-6 signaling in macrophages plays a role in their differentiation and proliferation. The JAK-STAT signaling pathway is involved in both cancer and autoimmune diseases and has been an attractive target for drug discovery in immunological diseases and cancer.

References

Tian S., et al., 1994, Blood. 84(6):1760-1764.
Zhong, Z., et al., 1994, Science. 264(5155):95-98.

Storage/Stability

Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, the use of validated and optimized media from BPS Bioscience is highly recommended. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin

Growth Medium 1N (BPS Bioscience #79801): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin, and 0.5 µg/ml of Puromycin

Assay Medium: Thaw Medium 1 (BPS Bioscience #60187)

Applications

Monitor STAT3 activity.
Screen for compound effect on the STAT3 signaling pathway.
Screen for inhibitors of STAT3-mediated IL-6 signaling.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only