STAT3 Luciferase Reporter THP-1 Cell Line

Catalog #

78498

$3,700 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The STAT3 Luciferase Reporter THP-1 Cell Line monitors the STAT3 (Signal Transducer and Activator of Transcription 3) signal transduction pathway. It contains a firefly luciferase reporter driven by STAT3 response elements located upstream of the minimal TATA promoter. After activation by cytokines or growth factors, the endogenous transcription factor STAT3 binds to the DNA response elements, inducing transcription of the luciferase reporter gene.

These cells respond to IL-6 (Interleukin 6), to G-CSF (Granulocyte Colony-Stimulating Factor) and to IFN-γ (Interferon γ).

Figure 1: Illustration of STAT3 Luciferase Reporter THP-1 Cell Line reporter expression.
Created with BioRender.com

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Signal transducer and activator of transcription 3, Acute-phase response factor, APRF, IL-6 responsive cell line, IFNy responsive cell line, G-CSF responsive cell line, STAT-3

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Product Data Gallery

Dose response to human IL-6 in STAT3 Luciferase Reporter THP-1 cells.

Cytokine response in STAT3 Luciferase Reporter THP-1 cells.

Dose-dependent response to human G-CSF in STAT3 Luciferase Reporter THP-1 cells.

Inhibition of hIL-6-induced STAT3 Reporter Activity by JAK inhibitor CP 690,550 in STAT3 Luciferase Reporter THP-1 cells.

Inhibition of hIL-6-induced STAT3 Reporter Activity by anti-IL-6 Receptor Antibody in STAT3 Luciferase Reporter THP-1 cells.

Inhibition of G-CSF-induced STAT3 Reporter Activity by anti-G-CSF Receptor Antibody in STAT3 Luciferase Reporter THP-1 cells.

Product Info

Storage and Usage

Citations

Host Cell Line

THP-1, monocyte, suspension

Supplied As

Each vial contains >1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 8	BPS Bioscience #79652
Growth Medium 8A	BPS Bioscience #79653

Materials Required for Cellular Assay

Name	Ordering Information
Thaw Medium 8	BPS Bioscience #79652
Recombinant Human IL-6 Protein	R&D Systems #206-IL
IFN-γ	R&D Systems #285-IF
Pan-JAK inhibitor CP 690,550	Cayman #11598
Human IL-6R alpha Antibody	R&D Systems #MAB227
Recombinant Human G-CSF Protein	R&D Systems #214-CS
Anti-GCSFR Antibody (Anumigilimab Biosimilar)	ProteoGenix #PX-TA1776
White, clear-bottom cell culture plate, 96-well
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
Luminometer

UniProt #

P40763

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

STAT3 (Signal Transducer and Activator of Transcription 3) is a transcription factor expressed in diverse cell types. STAT3 is phosphorylated by JAK (Janus Kinases) and gets translocated to the nucleus. While it was initially described as an acute phase response factor in the context of IL-6 signaling, STAT3 has since been identified as a downstream effector of many cytokines. STAT3-mediated IL-6 signaling in macrophages plays a role in their differentiation and proliferation. The JAK-STAT signaling pathway is involved in both cancer and autoimmune diseases and has been an attractive target for drug discovery in immunological diseases and cancer.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture

Thaw Medium 8 (BPS Bioscience #79652): RPMI 1640 medium supplemented with 10% heat-inactivated FBS, 1% Penicillin/Streptomycin

Growth Medium 8A (BPS Bioscience #79653): RPMI 1640 medium supplemented with 10% heat-inactivated FBS, 1% Penicillin/Streptomycin plus 1 μg/ml of Puromycin.

Media Required for Functional Cellular Assay

Thaw Medium 8 (BPS Bioscience #79652): RPMI 1640 medium supplemented with 10% heat-inactivated FBS, 1% Penicillin/Streptomycin

Applications

Monitor STAT3 activity in a monocyte cell line (THP1).
Screen for effects of compounds on the STAT3 signaling pathway.
Screen for inhibitors of STAT3-mediated IL-6 signaling.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

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STAT3 Luciferase Reporter THP-1 Cell Line

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