JAK1, GST-tag Recombinant

Catalog #

40449

$375 *

Size: 10 µg

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Human JAK1 (Janus kinase 1), also known as JTK3, GenBank Accession No. NM_002227, a.a. 866-1154(end) with N-terminal GST-Tag, MW= 59 kDa, expressed in Sf9 cells via a baculovirus expression system.

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Synonyms

JAK1, Janus kinase-1, Just Another Kinase 1, JTK3, jak

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥63%

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl pH 8.0, 427 mM NaCl, 1.3 mM KCl, , 0.02% Tween 20, 3 mM DTT, 14 mM glutathione and 50% glycerol.

60 kDa

Amino Acids

866-1154 (end)

Specific Activity

≥80 pmole/min/ug

Genbank #

NM_002227

UniProt #

P23458

Tag(s)

N-terminal GST-tag

References

1. Rodig, S.J., et al. (1998). Cell 93(3): 373–8.
2. Haan, C., et al. (2001). J. Biol. Chem. 276 (40): 37451–8.
3. Miyazaki, T., et al. (1994). Science 266 (5187): 1045–7

Storage/Stability

At least 6 months at –80°C

Assay Conditions

Assay was done in Kinase buffer containing 2 mM DTT using IRS-1 derived peptide substrate (0.1 mg/ml) and 20 µM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Cytoplasmic Tyr

Regulatory Status

Research Use Only

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JAK1, GST-tag Recombinant

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