Enhanced GFP Lentivirus (Puromycin) (Discontinued)

Catalog #

79979

$835 *

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Description

Enhanced green fluorescent protein (eGFP) is a modified (F64L and S65T mutations) version of the native GFP protein isolated from jellyfish (Aequorea victoria), with increased fluorescence and more efficient folding. The Enhanced GFP Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types mammalian cells, including primary and non-dividing cells. These viruses constitutively express eGFP under a CMV promoter (Figure 1). eGFP expression and transduction efficiency can easily be verified and optimized via fluorescence microscopy or flow cytometry. eGFP has an excitation wavelength of 488 nm, an emission wavelength of 509 nm, and extinction coefficient of 55,000 M^-1cm-1.

79979

Figure 1. Schematic of the lenti-vector used to generate the eGFP lentivirus

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Synonyms

VSV-G pseudotyped lentiviral particle, 79703

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Product Info

Storage and Usage

Citations

Supplied As

Two vials (2 x 500 μl) of eGFP lentivirus at a titer ≥1 x 10⁷TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Materials Required But Not Supplied

• HEK293 growth medium or use Thaw Medium 1 (BPS Bioscience #60187): MEM supplemented with 10% FBS, 1% non-essential amino acids (Hyclone #SH30238.01), 1 mM Na pyruvate (Hyclone #SH30239.01), 1% Penicillin/Streptomycin (Hyclone #SV30010.01).
• Polybrene (Millipore, #TR-1003-G)
• 6-well and T75 tissue culture-treated assay plates
• Flow cytometer or fluorescence microscope

Formulation

The lentiviruses were produced from HEK293T cells. Supplied in medium containing 90% DMEM + 10% FBS.

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Enhanced GFP Lentivirus (Puromycin) (Discontinued)

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