NFAT eGFP Reporter Lentivirus

Catalog #
79922
$910 *
Size: 500 µl x 2
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Description

The NFAT eGFP Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain an enhanced GFP gene driven by the NFAT response element located upstream of the minimal TATA promoter (Figure 1). After transduction, activation of the NFAT signaling pathway in the target cells can be monitored by examining eGFP expression.

Figure 1. Schematic of the lenti-vector used to generate the NFAT eGFP reporter lentivirus

Figure 1. Schematic of the lenti-vector used to generate the NFAT eGFP reporter lentivirus

Product Info
Storage and Usage
Citations
Supplied As
Two vials (500 μl x 2) of NFAT eGFP reporter lentivirus at a titer 1 x 107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Materials Required But Not Supplied

• Jurkat cells (ATCC # TIB-152)
• Anti-CD3 agonist antibody (BPS Bioscience, #71274)
• Jurkat growth medium (Thaw Medium 2, BPS Bioscience, #60184)
• Polybrene (Millipore, #TR-1003-G)
• 96-well tissue culture treated white clear-bottom assay plate (Corning, #3610)

Formulation

The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS.