NFAT Luciferase-eGFP Reporter Jurkat Cell Line

Catalog #

78662

$2,195 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Recombinant Jurkat T cells expressing both firefly luciferase and enhanced GFP (eGFP) under the control of NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway can be monitored by examining either firefly luciferase or eGFP expression.

Interested in screening and profiling inhibitors or activators of NFAT-mediated signaling pathways without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

Product Info

Storage and Usage

Citations

Host Cell Line

Jurkat (clone E6-1), human T lymphoblast, suspension

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of cell freezing medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Name	Ordering Information
Thaw Medium 2	BPS Bioscience #60184
Growth Medium 2E	BPS Bioscience #79638

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

The Nuclear Factor of Activated T Cells (NFAT) family of transcription factors plays an important role in mediating the immune response. T cell activation through the T cell synapse results in calcium influx, which is required to facilitate actin organization and interactions at the synapse. Increased intracellular calcium levels activate the calcium-sensitive phosphatase Calcineurin, which rapidly dephosphorylates the serine-rich region (SRR) and SP-repeats in NFAT proteins. This results in a conformational change that exposes a nuclear localization signal (NLS) promoting NFAT nuclear translocation and inducing gene expression, including various cytokines (IL-2, IL-3, IL-4, and TNF-alpha). Members of the NFAT family have been found in many tissue types, including the heart, skeletal muscle, and brain. These transcription factors are known to be highly involved in the pathological processes of inflammation and cancer. This reporter cell line is designed to monitor T cell activation or inhibition through various checkpoint inhibitors. It can be used as a control or parental cell line to co-express various immune checkpoint inhibitors, such as PD1.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture

Thaw Medium 2 (BPS Bioscience #60184): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 2E (BPS Bioscience #79638): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 0.5 µg/ml Puromycin.

Media Required for Functional Cellular Assay

Thaw Medium 2 (BPS Bioscience #60184): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Applications

Screen for activators or inhibitors of NFAT signaling pathway
Determine T cell activation through T Cell Receptor (TCR) or Chimeric Antigen Receptor (CAR)

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only