NFAT Luciferase-RFP Reporter Lentivirus

Only %1 left

Catalog #

78617

As low as $835 *

Cat # Size Price*

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

The NFAT Luciferase-RFP Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase and RFP (Red Fluorescent Protein) cassette driven by the NFAT response element located upstream of the minimal TATA promoter and a hygromycin or puromycin selection gene to generate stable clones. After transduction, activation of the NFAT signaling pathway in the target cells can be monitored by measuring the luciferase activity or RFP expression. RFP fluoresces red-orange when excited; it has an excitation wavelength of 553 nm, and an emission wavelength of 574 nm.

Schematic of the lenti-vector used to generate the NFAT luciferase-RFP reporter lentivirus (hygromycin).

Schematic of the lenti-vector used to generate the NFAT luciferase-RFP reporter lentivirus (puromycin).

●

Product Data Gallery

Product Info

Storage and Usage

Citations

Supplied As

Two vials (500 µl x 2) of lentivirus at a titer >10⁷ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Materials Required But Not Supplied

Name	Ordering Information
Jurkat cells	ATCC # TIB-152
Anti-CD3 agonist antibody	BPS Bioscience #71274
Thaw Medium 2	BPS Bioscience #60184
Polybrene	Millipore, #TR-1003-G
96-well tissue culture, clear-bottom, white plate	Corning, #3610
One-Step luciferase assay system	BPS Bioscience #60690
Luminometer

Formulation

The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.

Background

NFAT (Nuclear factor of activated T-cells) is a family of transcription factors that has an important function in immune responses, for example by inducing the expression of various cytokines (such as IL-2-3-4 and TNF-alpha) in T cells. Members of the NFAT family have been found in many tissue types including heart, skeletal muscle and brain cells. Through their role in the immune system, NFATs are involved in inflammation and these transcription factors are considered promising therapeutic targets for a variety of diseases.

NFAT is regulated by Ca²⁺ and the Ca²⁺/calmodulin-dependent serine phosphatase, calcineurin. NFAT proteins are phosphorylated and reside in the cytoplasm in resting cells; upon stimulation, they are dephosphorylated by calcineurin, translocate to the nucleus, and induce gene expression.

Storage/Stability

Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.

Applications

Screen for activators or inhibitors of NFAT signaling pathways in transduced target cells.
Generate NFAT Luciferase-RFP Reporter stable cell line (hygromycin or puromycin resistant).

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Biosafety
The lentiviruses are produced with the third generation SIN (self-inactivation) lentivector which
ensures self-inactivation of the lentiviral construct after transduction and after integration into the genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal and are not present in the lentivirus particle. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS Bioscience recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.

Warnings

Avoid repeated freeze-thaw cycles.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

NFAT Luciferase-RFP Reporter Lentivirus

PRODUCTS

SERVICES

RESOURCES

COMPANY