NFAT Reporter (Luciferase) – THP-1 Cell Line

Catalog #

78320

$1,810 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The NFAT reporter (Luciferase)-THP-1 cell line is designed for monitoring the NFAT (nuclear factor of activated T-cells) signaling pathway in THP-1 cells by measuring luciferase activity. It contains a firefly luciferase gene driven by the NFAT response element located upstream of the minimal TATA promoter. Upon activation by NFAT activators such as Ionomycin, endogenous NFAT transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene.

Interested in screening and profiling inhibitors, or activators of NFAT-mediated signaling pathways without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

nuclear factor of activated T cells, T cells transcription factor, NFAT1, NFATP, NFAT2; NFATc, NFATC1, NFATC2

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Product Data Gallery

NFAT activation in NFAT reporter (Luciferase)-THP-1 cells

Cyclosporin A inhibits NFAT signaling pathway activated by Ionomycin/PMA

Product Info

Storage and Usage

Citations

Host Cell Line

THP-1 Human leukemia monocytic cell line. Non-adherent cells.

Supplied As

2 vials: Each vial contains >1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Materials Required for Cell Culture

Name	Ordering Information
Thaw Medium 2	BPS Bioscience, #60184
Growth Medium 2M	BPS Bioscience, #78181

Materials Required for Cellular Assay

Name	Ordering Information
Ionomycin	Sigma, #I3909
PMA	Sigma-Aldrich, #P8139
Cyclosporin A	Cayman, #12088
Assay Medium: Thaw Medium 2	BPS Bioscience, #60184
Growth Medium 2M	BPS Bioscience, #78181
96-well tissue culture treated white clear-bottom assay plate	Corning, #3610
ONE-Step™ luciferase assay system	BPS Bioscience, #60690
Luminometer

Formulation

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 2 (BPS Bioscience, #60184): RPMI1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin

Growth Medium 2M (BPS Bioscience, #78181): RPMI1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 1 μg/ml of Puromycin

Assay Medium: Thaw Medium 2 (BPS Bioscience, #60184)

Mycoplasma Testing

The cell line has been screened using the PCR-based Venor®GeM Mycoplasma Detection kit (Sigma-Aldrich, #MP0025) to confirm the absence of Mycoplasma species.

References

1. Clipstone NA, Crabtree GR. Nature. 1992 Jun 25;357(6380):695-7.
2. Lyakh, L., et al. Mol Cell Biol. 1997 May;17(5):2475-84.

Storage/Stability

Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Instructions for Use

See protocol for detailed instructions

Applications

- Monitor NFAT signaling pathway activity.
- Screen for compound activity on the NFAT signaling pathway.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only