NF-κB Luciferase Reporter Lentivirus

Catalog #

79564

$875 *

Size: 500 µl x 2

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Bulk/Custom

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Description

The NF-κB Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter (Figure 1). After transduction, activation of the NF-κB signaling pathway in the target cells can be monitored by measuring the luciferase activity.

Lentivirus Reporter Map

Figure 1. Schematic of the lenti-vector used to generate the NF-κB luciferase reporter lentivirus

This product has been cited 7 times.

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Synonyms

nfkb, nf-kb

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Product Info

Storage and Usage

Citations7

Formulation

The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS.

References

1. Pessara, U., Koch, N. (1990) Tumor necrosis factor alpha regulates expression of the major histocompatibility complex class II-associated invariant chain by binding of an NF-κB-like factor to a promoter element. Mol Cell Biol. 10(8):4146-4154.

2. Baeuerle, P.A. (1998) Pro-inflammatory signaling: last pieces in the NF-κB puzzle? Curr Biol. 8(1):R19-R22.

Storage/Stability

Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.

Instructions for Use

See product datasheet

Shipping Temperature

-80°C

Warnings

The lentiviruses are produced with the third generation SIN (self-inactivation) lentivector which ensures self-inactivation of the lentiviral construct after transduction and integration into the genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal.

Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

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NF-κB Luciferase Reporter Lentivirus

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