NF-κB Reporter (Luc) – NIH/3T3 Cell line
The NF-κB reporter (Luc)-NIH-3T3 cell line is designed for monitoring nuclear factor Kappa B (NF-κB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene.
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This product has been cited 2 times.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
Materials Required for Cell Culture
| Name | Ordering Information |
| Thaw Medium 5 | BPS Bioscience #60182 |
| Growth Medium 5A | BPS Bioscience #79534 |
Materials Required for Cellular Assay
| Name | Ordering Information |
| hTNFα | R&D Systems 210-TA |
| QNZ (EVP4593) or other suitable inhibitor control | Selleckchem #S4902 |
| Assay Medium: Thaw Medium 5 | BPS Bioscience #60182 |
| Growth Medium 5A | BPS Bioscience #79534 |
| 96-well tissue culture treated white clear-bottom assay plate | Corning #3610 |
| ONE-Step™ luciferase assay system | BPS Bioscience #60690 |
| Luminometer |
The cell line has been screened using the PCR-based Venor®GeM Mycoplasma Detection kit (Sigma-Aldrich, #MP0025) to confirm the absence of Mycoplasma species.
1. Pessara U, Koch N (1990) Tumor necrosis factor alpha regulates expression of the major histocompatibility complex class II-associated invariant chain by binding of an NF-κB-like factor to a promoter element. Mol Cell Biol. 10(8):4146-4154.
2. Baeuerle PA (1998) Pro-inflammatory signaling: last pieces in the NF-κB puzzle? Curr Biol. 8(1):R19-R22.
Application Reference(s):
1. New software for automated cilia detection in cells (ACDC),(2019)
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