NF-κB Luciferase Reporter A549 Cell Line

Catalog #

60625

$1,915 *

Size: 2 vials

Qty

Bulk/Custom

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Description

NF-κB Luciferase Reporter A549 Cell Line is an A549 cell line expressing the NF-κB (nuclear factor kappa0light-chain-enhancer of activated B cells) luciferase reporter. The firefly luciferase reporter is controlled by 4 copies of NF-κB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-κB transcription factors bind to the DNA response elements to induce transcription of the luciferase reporter. This cell line has been validated for NF-κB stimulation by TNFα (tumor necrosis factor α) and LIGHT (tumor necrosis factor superfamily member 14 or TNFSF14).

Interested in screening and profiling inhibitors, blocking antibodies, or activators of NF-κB-mediated signaling without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

This product has been cited 3 times.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

NF-κB cell line, NFkappaB cell line, luciferase reporter, NF-kB cell line, NFkB cell line, nfkb, nf-kb

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Product Data Gallery

Dose response curve of NF-κB Luciferase Reporter A549 Cell Line to TNFα

NF-κB Luciferase Reporter A549 Cell Line response to LIGHT

Dose response curve of NF-κB Luciferase Reporter A549 Cell Line to the inhibitor IKK-16.

Product Info

Storage and Usage

Citations3

Host Cell Line

Human alveolar vassal carcinoma cell line. Adherent epithelial cells.

Species

Human

Supplied As

Each vial contains ≥1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Materials Required For Cell Culture

Name	Ordering Information
Thaw Medium 6	BPS Bioscience #60183
Growth Medium 6B	BPS Bioscience #79657

Materials Required for Cellular Assay

Name	Ordering Information
hTNFα (carrier free)	R&D Systems 210-TA
LIGHT, His-Tag (Human) Recombinant	BPS Bioscience #71266
IKK-16 dihydrochloride (or other suitable inhibitor control)	Sigma SML 1138
Assay Medium: Thaw Medium 6	BPS Bioscience #60183
Growth Medium 6B	BPS Bioscience #79657
96-well tissue culture treated white clear-bottom assay plate	Corning #3610
ONE-Step™ luciferase assay system	BPS Bioscience #60690
Luminometer

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

NF-κB signaling plays a pivotal role in regulating cell development and immune homeostasis, by regulating DNA transcription, cytokine production and apoptosis. It is ubiquitously present in almost all mammalian cells, and responds to cellular stress signals, such as stress, free radicals, UV radiation and bacteria and virus. Activation of NF-κB through tumor necrosis factor receptors (TNFR) or the TNFR superfamily (tumor necrosis factor superfamily member 14 or TNFSF14). Activation of NF-κB enhances cell inflammation and prevents apoptosis, which contribute to tumor development. The A549 lung epithelial cell line is ideal as in vitro lung disease model for high throughput screening of inhibitors of upstream effectors of the NF-κB signaling pathway.

References

Cherfilis-Vicini J., al., 2010 J. Clin. Invest. 120: 1285-1297.
Chen W., al., 2011 Front. Biosci. 16: 1172-1185.
Callister M.E., al., 2008 Br. J. Pharm. 155: 661-672.
Schmeck B., al., 2007 Eur. Respir. J. 29: 25-33

Storage/Stability

Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 6 (BPS Bioscience #60183): DMEM medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 6B (BPS Bioscience #79657): DMEM medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 1 mg/ml Geneticin.

Media Required for Functional Cellular Assay
Thaw Medium 6 (BPS Bioscience #60183): DMEM medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Applications

Monitor NF- κB signaling pathway activity.
Screen for compound activity on the NF-κB signaling pathway

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only