NF-κB Luciferase Reporter Raw 264.7 Cell Line

Catalog #

79978

$2,045 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

NF-κB Luciferase Reporter Raw 264.7 Cell Line is a Raw 264.7 cell line expressing firefly luciferase driven by four copies of the NF-kB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. This cell line has been functionally validated and responds to TNFα and RANKL

Interested in screening and profiling inhibitors or activators of NF-κB-mediated signaling without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

NF-kB reporter, NFkappaB reporter, luciferase reporter, NF-kB reporter, NFkB reporter, nfkb 264.7, 264.7 raw, NF-kB, nfkb, nf-kb

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Product Data Gallery

Dose response curve of NF-κB Luciferase Reporter Raw 264.7 Cell Line to mouse RANKL.

Dose response curve of NF-κB Luciferase Reporter Raw 264.7 Cell Line to mouse TNFα.

Product Info

Storage and Usage

Citations

Host Cell Line

Raw 264.7, murine macrophage cell line, adherent.

Supplied As

Each vial contains >1x 10⁶ cells in 1 ml of cell freezing medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 11	BPS Bioscience #79976
Growth Medium 11A	BPS Bioscience #79977

Materials Used in the Cellular Assay

Name	Ordering Information
Mouse RANKL	R&D Systems #462-TEC-010
Mouse TNFα	R&D Systems #410-MT
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
White clear-bottom 96-well cell culture plate
Luminometer

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

The role of NF-κB (nuclear factor-κB) activation is well-characterized in canonical (classical) and noncanonical (alternative) signaling pathways of inflammation. Two major forms of innate immune sensors are Toll-like receptors (TLR) and NOD/CATERPILLAR proteins. Mutations in NOD2 (nucleotide-binding oligomerization domain-containing protein 2) have been linked to chronic autoinflammatory and autoimmune diseases, such as Crohn’s disease and Blaus syndrome. Studying the canonical and noncanonical NF-κB pathways and the influence of TLR pathways and NOD2 mutations can further our understanding of autoimmune regulation. Raw 264.6 is a murine macrophage cell line useful for studies involving immunoreactivity. The use of a luciferase reporter allows for easy read outs in cellular assays.

References

Peninnger, J. M., et al., 2006 Trends Mol. Med. 12(1):17-25.
Baeuerle, P.A., 1998 Curr Biol. 8(1):R19-R22.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 11 (BPS Bioscience #79976): DMEM medium supplemented with 10% heat-inactivated FBS, 1% GlutaMAX, 1% Penicillin/Streptomycin.

Growth Medium 11A (BPS Bioscience #79977): DMEM medium supplemented with 10% heat-inactivated FBS, 1% GlutaMAX, 1% Penicillin/Streptomycin plus and 700 μg/ml of Genecticin.

Media Required for Functional Cellular Assay
Thaw Medium 11 (BPS Bioscience #79976): DMEM medium supplemented with 10% heat-inactivated FBS, 1% GlutaMAX, 1% Penicillin/Streptomycin.

Instructions for Use

See assay protocol for detailed instructions.

Applications

Screen for activators or inhibitors of the NF-κB signaling pathway.
Screen for activators or inhibitors of the RANKL/RANK pathway.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

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NF-κB Luciferase Reporter Raw 264.7 Cell Line

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