NF-κB Reporter (Luc) - HEK293 Recombinant Cell line
Catalog #
60650
$1,430*
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Description
NF-κB Reporter (Luc) HEK293 Cell Line is a HEK293 cell designed to monitor nuclear factor Kappa B (NF-κB) activity. It contains a firefly luciferase reporter driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or agonists of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter. The cell line has been functionally validated in response to human TNF-α, IL-1β, and IL-17.
Interested in screening and profiling inhibitors, blocking antibodies, or activators of NF-κB-mediated signaling without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.
The cell line has been screened to confirm the absence of Mycoplasma species.
References
Pessara U. and Koch N., 1990 Mol Cell Biol. 10(8):4146-4154. Baeuerle P.A., 1998 Curr Biol. 8(1): R19-R22. Takada Y., et al., 2005 J Biol Chem. 280(17): 17203-17212.
Storage/Stability
Cells will arrive in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.
Growth Media
For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.
Media Required for Cell Culture Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin
Growth Medium 1C (BPS Bioscience #79532): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin and 50 µg/ml of Hygromycin B
Media Required for Functional Cellular Assay Assay Medium: Thaw Medium 1 (BPS Bioscience #60187)
Applications
Monitor NF-κB activity.
Screen for compound activity on the NF-κB signaling pathway.
Shipping Temperature
-80°C
Notes
License Disclosure Visit bpsbioscience.com/license for the label license and other key information about this product.