NF-κB Reporter (Luc) - HEK293 Recombinant Cell line

Catalog #

60650

$1,430 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

NF-κB Reporter (Luc) HEK293 Cell Line is a HEK293 cell designed to monitor nuclear factor Kappa B (NF-κB) activity. It contains a firefly luciferase reporter driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or agonists of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter. The cell line has been functionally validated in response to human TNF-α, IL-1β, and IL-17.

Interested in screening and profiling inhibitors, blocking antibodies, or activators of NF-κB-mediated signaling without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

This product has been cited 28 times.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

NF-κB cell line, NFkappaB cell line, luciferase reporter, NF-kB cell line, NFkB cell line, nfkb, nf-kb, TNFa reporter, IL-17 reporter, IL-1b reporter, IKK-16 responsive cell line, TNFa responsive cell line, anti-IL17-A neutralizing antibody responsive cel

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Product Data Gallery

Dose response curve of NF-κB Reporter (Luc) HEK293 Cell Line to hTNFα

Dose response of NF-κB Reporter (Luc) HEK293 Cell Line to IL-1β.

Dose response curve of NF-κB reporter (Luc) HEK293 Cell Line to IL-17A.

Inhibition of TNFα-induced NF-κB activity by the NF-κB inhibitor IKK-16 dihydrochloride in the NF-κB Reporter (Luc) HEK293 Cell Line.

Inhibition of IL-1β-induced NF-κB activity by the NF-κB inhibitor IKK-16 dihydrochloride in the NF-κB Reporter (Luc) HEK293 Cell Line.

Inhibition of IL-17A-induced NF-κB activity by the NF-κB inhibitor IKK-16 dihydrochloride in the NF-κB Reporter (Luc) HEK293 Cell Line.

Inhibition of IL-17A-induced NF-κB activity by Brodalumab, an anti-IL-17-receptor antibody, in the NF-κB Reporter (Luc) HEK293 Cell Line.

Inhibition of IL-17A-induced NF-κB activity by Anti-IL-17 Neutralizing Antibody in the NF-κB Reporter (Luc) HEK293 Cell Line.

Product Info

Storage and Usage

Citations28

Host Cell Line

HEK293, epithelial-like cells, adherent

Species

Human

Supplied As

Each vial contains ˃1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Materials Required for Cell Culture

Name	Ordering Information
Thaw Medium 1	BPS Bioscience #60187
Growth Medium 1C	BPS Bioscience #79532

Materials Required for Cellular Assay

Name	Ordering Information
Recombinant Human TNF-alpha Protein (hTNFα)	R&D Systems 210-TA
Human Interleukin-1 beta Recombinant (IL-1β)	BPS Bioscience #90168
hIL-17A, Avi-Tag Recombinant	BPS Bioscience #91014
Assay Medium: Thaw Medium 1	BPS Bioscience #60187
IKK-16 dihydrochloride: inhibitor of NF-κB activation	Sigma #SML1138
Brodalumab (Anti-IL-17R Antibody)	MedChemExpress #HY-P9925
Anti-IL-17A Neutralizing Antibody	BPS Bioscience #91015
96-well tissue culture-treated white clear-bottom assay plate	Corning #3610
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
Luminometer

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

References

Pessara U. and Koch N., 1990 Mol Cell Biol. 10(8): 4146-4154.
Baeuerle P.A., 1998 Curr Biol. 8(1): R19-R22.
Takada Y., et al., 2005 J Biol Chem. 280(17): 17203-17212.

Storage/Stability

Cells will arrive in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin

Growth Medium 1C (BPS Bioscience #79532): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin and 50 µg/ml of Hygromycin B

Media Required for Functional Cellular Assay
Assay Medium: Thaw Medium 1 (BPS Bioscience #60187)

Applications

Monitor NF-κB activity.
Screen for compound activity on the NF-κB signaling pathway.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only