GITR / NF-κB Luciferase Reporter - Jurkat Cell Line

Catalog #

60546

$10,175 *

Size: 2 vials

Qty

Bulk/Custom

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Description

This cell line expresses a surface human GITR (glucocorticoid-induced TNFR family related gene; TNFRSF18; CD357) and an NF-ĸB luciferase reporter construct that are stably integrated into the genome of Jurkat T-cells. The firefly luciferase gene is controlled by 4 copies of NF-kB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-ĸB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene.

Interested in screening and profiling inhibitors, blocking antibodies, or activators of GITR without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

NFKB, GITR, NF-KB, nfkb, nf-kb

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Product Data Gallery

Expression of GITR protein in GITR / NF-ĸB-Luciferase Reporter - Jurkat cells validated by flow cytometry

Analysis of GITR NF-ĸB / Jurkat reporter activity in response to human GITRL

GITRL stimulates GITR NF-ĸB Luciferase Jurkat, not NF-kB Luciferase

GITR NFkB Reporter Cells recognizes GITRL presented in the CHO-K1 cells

Dose response of anti-GITR agonist antibody on GITR/NFkB Jurkat cells cocultured with FcGR2B CHO K1 cells

Product Info

Storage and Usage

Citations

Host Cell Line

Jurkat

Supplied As

Each vial contains ~2 x 106 cells in 1 ml of 10% DMSO in FBS.

UniProt #

Q9Y5U5

Mycoplasma Testing

This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Cat. #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Cat. #LT07-518) was used as a positive control.

Background

Glucocorticoid-induced TNFR-related protein (GITR) is a member of the TNFR superfamily, expressed in a number of cells including T cells, natural killer (NK) cells and antigen-presenting cells (APC). GITRL, GITR’s natural ligand, is expressed mainly by APCs and GITR:GITRL interaction is important for activation of the immune system. Agonistic antibodies targeting GITR are actively being pursued as potential immuno-oncology therapies.

References

Esparza, E.M. and Arch, R.H. (2005) Glucocorticoid-Induced TNF Receptor, a Costimulatory Receptor on Naïve and Activated T Cells, Uses TNF Receptor-Associated Factor 2 in a Novel Fashion as an Inhibitor of NF-kB Activation. J Immunology. 174: 7875-7882.

Storage/Stability

Store in liquid nitrogen immediately upon receipt.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 2 (BPS Cat. #60184): RPMI1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin

Growth Medium 2C (BPS Cat. #79592): RPMI1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 100 µg/ml Hygromycin and 1 mg/ml G418

Applications

The GITR NF-ĸB-luciferase/Jurkat cell line is suitable for monitoring the activity of NF-kB signaling in response to GITR ligand (GITRL).

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only