GITRL:GITR[Biotinylated] Inhibitor Screening Assay Kit
The GITR:GITRL [Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of GITR:GITRL signaling. This kit comes in a convenient 96 well format, with biotin-labeled GITR (CD357), purified GITRL, streptavidin-labeled HRP, and Immunobuffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled GITR by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, GITRL is coated on a 96-well plate. Next, GITR is incubated with GITRL on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.
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| Catalog Number |
Component |
Amount |
Storage |
|
| 71256 | GITR, Biotin-labeled | 2 µg | -80°C | Avoid freeze/ thaw cycles! |
| 71190 | GITRL-His | 10 µg | -80°C | |
| 79742 | Streptavidin-HRP | 15 µl | +4°C | |
| 79311 | 3x Immunobuffer 1 | 50 ml | -20°C | |
| 79728 | Blocking Buffer 2 | 50 ml | +4°C | |
| 79670 | ELISA ECL substrate A (transparent bottle) |
6 ml | RT | |
| ELISA ECL substrate B (brown bottle) |
6 ml | RT | ||
| 79699 | White 96-well microplate | 1 | +4°C | |
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