GITRL, His-tag (Human) Recombinant

Catalog #

71190

$320 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Human glucocorticoid-induced TNF-related ligand (GITRL), also known as AITRL, CD357L, or tumor necrosis factor ligand superfamily member 18 (TNFSF18). GenBank Accession No. NM_005092, a.a. 72-199 with an N-terminal His-tag, expressed in a HEK293 cell expression system. MW = 17.1 kDa. Note: this protein runs at a higher MW by SDSPAGE due to glycosylation.

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Synonyms

glucocorticoid-induced TNF-related ligand, AITRL, CD357L, tumor necrosis factor ligand superfamily member 18, TNFSF18

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

GITRL: GITR-biotin Binding

GITR: GITRL inhibition curve using a TR-FRET assay (#79054). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

17.1 kDa

Endotoxin Level

<1 EU/μg

Amino Acids

72-199

Genbank #

NM_005092

UniProt #

Q9UNG2

Tag(s)

N-terminal His-tag

References

1. Shevach, E.M. et al., Nat. Rev. Immunol. 2006; 6: 613-618.
2. Hanabuchi, S. et al., Blood. 2006; 107(9): 3617-3623.

Storage/Stability

>6 months at –80°C.

Assay Conditions

GITR-biotin binding to GITRL was confirmed using the GITRL: GITR Biotinylated] Inhibitor Screening Assay Kit (BPS Cat. #72061).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for studying protein binding and for screening small molecules and antibodies.

Shipping Temperature

-80°C

Notes

The aggregation state of this product has been confirmed to be <10%.

Warnings

Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 μg/ml in PBS + glycerol and stored at -80°C.

Scientific Category

Immunotherapy & Cytokines

Regulatory Status

Research Use Only

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Datasheets/Protocols

71190_Lot140826-D.pdf

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GITRL, His-tag (Human) Recombinant

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