NF-κB eGFP Reporter Lentivirus

Catalog #

79926

$820 *

Size: 500 µl x 2

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The NF-κB eGFP Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. The particles contain an enhanced GFP gene driven by the NF-κB response element located upstream of the minimal TATA promoter (Figure 1). After transduction, activation of the NF-κB signaling pathway in the target cells can be monitored by examining eGFP expression.

Figure 1. Schematic of the lenti-vector used to generate the NF-κB eGFP reporter lentivirus

Figure 1. Schematic of the lenti-vector used to generate the NF-κB eGFP reporter lentivirus

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Synonyms

NF-kB reporter, NFkappaB pseudovirion, nfkb pseudovirus, NF-kB lenti, NFkB lentivirus

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Product Data Gallery

Product Info

Storage and Usage

Citations

Species

Replication incompetent HIV

Supplied As

Two vials (500 μl x 2) of NF-κB eGFP reporter lentivirus at a titer 1 x 10⁷ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Materials Required But Not Supplied

Name	Ordering Information
TNFα	Sigma #T0157-10UG
Thaw Medium 9	BPS Bioscience #79665
Polybrene	Millipore #TR-1003-G
96-well white clear-bottom assay plate	Corning #3610
Negative Control eGFP Reporter Lentivirus	BPS Bioscience #79927
ONE-STEP Luciferase Assay System	BPS Bioscience #60690

Formulation

The lentiviruses were produced from HEK293T cells. Supplied in medium containing 90% DMEM + 10% FBS.

Storage/Stability

Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Biosafety
The lentiviruses are produced with the SIN (self-inactivation) lentivector which ensures self-inactivation of the lentiviral construct after transduction and integration into the genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

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NF-κB eGFP Reporter Lentivirus

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