NF-κB Reporter (Luc) - HCT116 Recombinant Cell Line

Catalog #

60623

$1,095 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The NF-κB reporter (Luc)-HCT-116 cell line is designed to monitor nuclear factor Kappa B (NF-κB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or agonists of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene.

Interested in screening and profiling inhibitors, blocking antibodies, or activators of NF-κB-mediated signaling without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

NF-κB cell line, NFkappaB cell line, luciferase reporter, NF-kB cell line, NFkB cell line, nfkb, nf-kb

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Product Data Gallery

hTNFα dose response in NF-κB reporter (Luc)-HCT-116 cells

Inhibition of hTNFα-induced NF-κB activity by NF-κB inhibitor, IKK-16 dihydrochloride, in NF-κB reporter (Luc)-HCT-116 cells

Product Info

Storage and Usage

Citations

Host Cell Line

HCT-116

Supplied As

Each vial contains ~2 X 10⁶ cells in 1 ml of 90% FBS, 10% DMSO

Materials Required But Not Supplied

Materials Required for Cell Culture

Name	Ordering Information
Thaw Medium 7	BPS Bioscience #60185
Growth Medium 7A	BPS Bioscience #79543

Materials Required for Cellular Assay

Name	Ordering Information
hTNFα	R&D Systems #210-TA
Assay Medium: Thaw Medium 7	BPS Bioscience #60185
IKK-16 dihydrochloride: inhibitor of NF-κB activation	Sigma #SML1138
96-well tissue culture-treated white clear-bottom assay plate	Corning #3610
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
Luminometer

Format

Aqueous solution containing DMSO

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

References

1. Samuel T et.al. (2014) Variable NF-κB pathway responses in colon cancer cells treated with chemotherapeutic drugs. BMC Cancer 14: 599.
2. Arabi A et.al. (2012) Proteomic screen reveals Fbw7 as a modulator of the NF-κB pathway. Nature Communication
3: 976. 3. Clemo NK et.al. (2008) BAG-1 is up-regulated in colorectal tumour progression and promotes colorectal tumour cell survival through increased NF-κB activity. Carcinogenesis 29: 849.
4. Tukhvatulin AI et.al. (2011) An In Vitro and In Vivo Study of the ability of NOD1 Ligands to Activate the Transcriptional Factor NF-κB. Acta Naturae 3: 77.

Storage/Stability

Store in liquid nitrogen immediately upon receipt.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 7 (BPS Bioscience #60185): McCoy’s 5A medium with 10% FBS, 1% Penicillin/Streptomycin

Growth Medium 7A (BPS Bioscience #79543): McCoy’s 5A medium with 10% FBS, 1% Penicillin/Streptomycin plus 1 mg/ml Geneticin (G418)

Assay Medium: Thaw Medium 7 (BPS Bioscience #60185)

Instructions for Use

See product datasheet

Applications

• Monitor NF-κB signaling pathway activity in response to stimulants such as the cytokines TNFα and IL-1β, pathogen-associated molecular pattern (PAMP) (i.e. flagellin) or endogenous damage-associated molecular pattern (DAMP) molecules (i.e. NOD1 ligand) (see references).
• Screen for compound activity of the NF-κB signaling pathway

Shipping Temperature

dry ice

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only