Renilla Luciferase Lentivirus (G418 or Puromycin)

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79565

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Description

The Renilla Luciferase Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles constitutively express Renilla luciferase under a CMV promoter (Figure 1: 79565-P, Figure 2: 79565-G).

Figure 1. Schematic of the lenti-vector used to generate the Renilla lentivirus with puromycin selection (BPS Bioscience, #79565-P):

Figure 1. Schematic of the lenti-vector used to generate the Renilla lentivirus

Figure 2. Schematic of the lenti-vector used to generate the Renilla lentivirus with G418 selection (BPS Bioscience, #79565-G):

Figure 2. Schematic of the lenti-vector used to generate the Renilla luciferase lentivirus

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Synonyms

rluc

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Product Info

Storage and Usage

Citations

Supplied As

Two vials (500 μl x 2) of Renilla luciferase lentivirus at a titer ≥5 x 10⁶ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Formulation

The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS.

References

1. Pessara, U., Koch, N. (1990) Tumor necrosis factor alpha regulates expression of the major histocompatibility complex class II-associated invariant chain by binding of an NF-κB-like factor to a promoter element. Mol Cell Biol. 10(8):4146-4154.

2. Baeuerle, P.A. (1998) Pro-inflammatory signaling: last pieces in the NF-κB puzzle? Curr Biol. 8(1):R19-R22.

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Renilla Luciferase Lentivirus (G418 or Puromycin)

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