Firefly Luciferase Lentivirus (G418, Hygromycin and Puromycin)

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Catalog #

79692

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Description

The Firefly Luciferase Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types mammalian cells, including primary and non-dividing cells. This virus constitutively express firefly luciferase under a CMV promoter (Figure 1: Hygromycin, Figure 2: G418 and Figure 3: Puromycin).

Figure 1. Schematic of the lenti-vector used to generate the firefly luciferase lentivirus (Hygromycin)

Figure 2. Schematic of the lenti-vector used to generate the firefly luciferase lentivirus (G418)

Figure 3. Schematic of the lenti-vector used to generate the firefly luciferase lentivirus (Puromycin)

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Product Info

Storage and Usage

Citations

Species

Replication incompetent HIV

Supplied As

Two vials (500 μl x 2) of firefly luciferase lentivirus at a titer ≥5 x 10^6 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Formulation

The lentiviruses were produced from HEK293T cells. Supplied in medium containing 90% DMEM + 10% FBS.

References

Application References:
G418
1. Trans-portal hepatic infusion of cultured bone marrow-derived mesenchymal stem cells in a steatohepatitis murine model (2020)

Puromycin
1. Common dandelion (Taraxacum officinale) efficiently blocks the interaction between ACE2 cell surface receptor and SARS-CoV-2 spike protein D614, mutants D614G, N501Y, K417N and E484K in vitro (2021)

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Firefly Luciferase Lentivirus (G418, Hygromycin and Puromycin)

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