JAK2 (JH2 Domain) (V617F, W659A, W777A, F794H), His-Tag Recombinant

Catalog #

100124

$420 *

Size: 20 µg

Qty

Bulk/Custom

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Description

Recombinant human JAK2 (Janus Kinase 2) encompassing amino acids 536-812, containing the pseudo-kinase domain JH2 with mutations V617F, W659A, W777A and F794H. The construct contains a C-terminal His tag (6xHis). The protein was affinity purified.

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Synonyms

Janus kinase-2, Just Another Kinase 2, JAK-2, JTK10, Tyrosine-protein kinase JAK2

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Product Data Gallery

4-20% SDS-Page Coomassie Staining

JAK2-JH2 Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

JAK2 (V617F, W659A, W777A, F794H) (536-812-Th-His)

Mutation

V617F, W659A, W777A, F794H

Host Species/Expression System

Sf9

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.5mM TCEP, 20% glycerol

33 kDa

Amino Acids

536-812

Genbank #

NM_004972

UniProt #

O60674

Tag(s)

C-terminal His-tag

References

1. Tono, C., et al., Leukemia. 2005; 19(10): 1843-1844.

2. Goerttler, P.S., et al., Blood. 2005; 106(8): 2862-2864

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was done according to the JAK2-JH2 Pseudokinase Domain (W659A, W777A, F794H) Inhibitor Screening Assay Kit (BPS Bioscience #78209) with JAK2 at various concentrations.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase

Regulatory Status

Research Use Only

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JAK2 (JH2 Domain) (V617F, W659A, W777A, F794H), His-Tag Recombinant

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