STAT5 Luciferase Reporter Ba/F3 Cell Line

Catalog #

79772

$2,275 *

Size: 2 vials

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

The STAT5 (Signal Transducer and Activator of Transcription 5) Luciferase Reporter murine Ba/F3 cell line monitors STAT5-mediated signal transduction pathways. It contains a firefly luciferase gene driven by STAT5 response elements located upstream of a minimal TATA promoter. After activation by relevant cytokines or growth factors, endogenous STAT5 binds to the response elements, inducing transcription of the luciferase reporter gene.

This cell line responds to mouse interleukin-3 (IL-3).

Figure 1: Illustration of STAT5 Luciferase Reporter Ba/F3 Cell Line.
Created with BioRender.com

Interested in screening and profiling inhibitors, blocking antibodies, or activators of STAT5-mediated signaling without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

●

Synonyms

STAT-5

●

Product Data Gallery

Dose-dependent response of STAT5 Luciferase Reporter Ba/F3 Cell Line to mouse IL-3

Product Info

Storage and Usage

Citations

Host Cell Line

Ba/F3, mouse IL-3-dependent pro-B cell line, suspension

Supplied As

Each vial contains > 1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 8	BPS Bioscience #79652
Mouse IL-3	BPS Bioscience #90189
Growth Medium 8A	BPS Bioscience #79653

Materials Required for Cellular Assay

Name	Ordering Infromation
Assay Medium: Thaw Medium 8	BPS Bioscience #79652
Mouse IL-3	BPS Bioscience #90189
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
White, clear-bottom 96-well tissue culture plate	Corning #3610
Luminometer

UniProt #

P42229

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

Signal transducer and activator of transcription 5 (STAT5) includes the highly related proteins STAT5A and STAT5B. These transcription factors relay cytokine-induced signals from the membrane to the nucleus, regulating the expression of hundreds of specific target genes. Cytokine or growth factor binding to its receptor activates a kinase of the JAK (Janus Kinase) family, which then phosphorylates STAT5 to trigger its dimerization and nuclear translocation. STAT5 is involved in the development and function of B and T cells. Constitutive phosphorylation of STAT5 is often observed in cancer. The use of inhibitors of the JAK/STAT pathways are potential candidates for the treatment of inflammation related diseases.

References

Palacios R., et al., 1984 Nature, 309 (5964): 126-131.
Jaster R., et al., 1999 Cell Signal., 11(5): 331-335.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, the use of these validated and optimized media from BPS Bioscience is highly recommended. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 8 (BPS Bioscience #79652) and mouse IL-3: RPMI 1640 medium supplemented with 10% heat-inactivated FBS, 1% Penicillin/Streptomycin and 5 ng/ml mouse IL-3.

Growth Medium 8A (BPS Bioscience #79653) and mouse IL-3: RPMI 1640 medium supplemented with 10% heat-inactivated FBS, 1% Penicillin/Streptomycin, 1 µg/ml of Puromycin, and 5 ng/ml mouse IL-3.

Media Required for Functional Cellular Assay
Thaw Medium 8 (BPS Bioscience #79652): RPMI 1640 medium supplemented with 10% heat-inactivated FBS, 1% Penicillin/Streptomycin.

Instructions for Use

See assay protocol for detailed instructions.

Applications

Monitor STAT5 activity.
Screen and characterize compounds that regulate STAT5 activity.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only