STAT5 Luciferase Reporter U937 Cell Line
The STAT5 (Signal transducer and activator of transcription 5) Luciferase Reporter U937 cell line monitors STAT5-mediated signal transduction pathways in the human monocyte cell line U937. It contains a firefly luciferase gene driven by STAT5 response elements located upstream of the minimal TATA promoter. After activation by GM-CSF (Granulocyte-macrophage colony-stimulating factor), the endogenous transcription factor STAT5 binds to the response elements, inducing transcription of the luciferase reporter gene.
These cells respond to GM-CSF. Functional validation experiments showed that GM-CSF-induced luciferase activity was inhibited by anti-GM-CSF neutralizing antibody (Clone #BVD2-21C11).
Figure 1: Illustration of STAT5 Luciferase Reporter U937 Cell Line mechanism of action.
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Interested in screening and profiling inhibitors, blocking antibodies, or activators of STAT5 signaling pathways without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
Materials Required for Cell Culture
| Name | Ordering Information |
| Thaw Medium 8 | BPS Bioscience #79652 |
| Growth Medium 8A | BPS Bioscience #79653 |
Materials Required for Cellular Assay
| Name | Ordering Information |
| Recombinant Human GM-CSF | Biolegend #572903 |
| Anti-GM-CSF neutralizing antibody | Biolegend #502319 (clone #BVD2-21C11) |
| ONE-Step™ Luciferase Assay System | BPS Bioscience #60690 |
| White, clear-bottom 96-well tissue culture plate | Corning #3610 |
| Luminometer |
The cell line has been screened using the MycoAlert™ Mycoplasma Detection kit (Lonza, #LT07-218) to confirm the absence of Mycoplasma species.
GM-CSF is a cytokine that can stimulate the generation of myeloid cells such as granulocytes, neutrophils, and monocytes from its bone marrow precursors. GM-CSF has pro-inflammatory functions and is a therapeutic target in autoimmune disease. GM-CSF signals through JAK2/STAT5 and stimulates the expression of STAT5 target genes. STAT5 includes the highly related proteins STAT5A and STAT5B. These transcription factors mediate cytokine-induced signals from the membrane to the nucleus, regulating the expression of hundreds of specific target genes. Cytokine or growth factor binding to its receptor activates a kinase of the JAK (Janus Kinase) family, which then phosphorylates STAT5 to trigger its dimerization and nuclear translocation. Constitutive phosphorylation of STAT5 is often observed in cancer
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