ACE2, His-Tag, Eu-labeled Recombinant

Catalog #

100705

$320 *

Size: 100 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Recombinant human ACE2 (Angiotensin converting enzyme 2), encompassing amino acids 18-740. This construct contains a C-terminal His-tag (10xHis). The protein was affinity purified and labeled with trFluor™ Eu.

●

Synonyms

ACEH, Angiotensin converting enzyme 2, Angiotensin-converting enzyme homolog

●

Product Data Gallery

4-20% SDS-Page Coomassie Staining

ACE2-Eu Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

ACE2 (18-740-His)-(trFluor-Eu)

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution

Formulation

8 mM phosphate, 110 mM NaCl, 2.2 mM KCl, 20% glycerol

85 kDa + glycans

Amino Acids

18-740

Glycosylation

This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Genbank #

NM_021804

UniProt #

Q9BYF1

Tag(s)

C-terminal His-tag

Label

trFluor™ Eu (Ex/Em = 346nm / 617 nm) is a fluorophore with large Stokes shifts and an extremely long emission half-live. It is ideal for applications that require high sensitivity and for time-resolved fluorometry because a delay between excitation and the detection of emission minimizes interference. EU-labeled probes are characterized by a relatively high stability and are often insensitive to quenching. trFluor™ Eu is covalently and randomly conjugated to the target protein.

References

1. Keider, S., et al., Nephrology Dialysis Transplantation. 2007 22(2): 597-601.
2. Donoghue, M., et al., Circulation Research. 2002 Sep;87:e1-e9

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was performed according to the Spike S1-Biotin (SARS-CoV-2): ACE2 TR-FRET Assay Kit (BPS Bioscience #79949-1) with Spike S1-Biotin (BPS Bioscience #100679) enzyme titrated from 200 ng/rxn – 16 ng/rxn and ACE-Eu at 12.5 ng/rxn.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Coronavirus, COVID-19

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

ACE2, His-Tag, Eu-labeled Recombinant

PRODUCTS

SERVICES

RESOURCES

COMPANY