JNK2, GST-Tag Recombinant

Catalog #

40113

$410 *

Size: 10 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Recombinant Human JNK2 also known as MAPK9 (mitogen-activated protein kinase 9), full length this construct contains an N-terminal GST-tag. The recombinant protein is kinase active and is affinity purified.

●

Synonyms

MAPK9, SAPK, p54a, JNK2A, JNK2B, PRKM9, JNK-55, JNK2BETA, p54aSAPK, JNK2ALPHA, JNK-2

●

Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

JNK2 (GST-Full Length)

Host Species/Expression System

Sf9

Purity

≥90%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol

70 kDa

Amino Acids

Full Length

Specific Activity

13 pmol/min/µg

Genbank #

NM_139069

UniProt #

P45984

Tag(s)

N-terminal GST-tag

Background

JNK2 is a member of the JNK family that acts as an integration point for multiple biochemical signals and is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK2 targets specific transcription factors, and mediates immediate-early gene expression in response to various cell stimuli. Both UV radiation and the proinflammatory cytokine TNFα can induce JNK2. JNK2 play a main role in the regulation of regional specific apoptosis (1) during early brain development and providing protection against autoimmune diabetes (2).

References

1. Kuan, C. et.al: The Jnk1 and Jnk2 protein kinases are required for regional specific apoptosis during early brain development. Neuron 22: 667-676, 1999.
2. Jaeschke, A. et.al: Disruption of the Jnk2 (Mapk9) gene reduces destructive insulitis and diabetes in a mouse model of type I diabetes. Proc. Nat. Acad. Sci. 102: 6931-6935, 2005.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Kinase activity was measured using P38 Sub synthetic peptide (IPTTPITTTYFFFKKK) substrate diluted in water to a final concentration of 1 mg/ml. Increasing amounts of kinase were mixed with a final concentration of 200 µg/ml peptide substrate in a buffer consisting of 5 mM MOPS pH 7.2, 5 mM MgCl₂, 2.5 mM β-glycerol-phosphate, 1 mM EGTA, 0.4 mM EDTA, 50 ng/µl BSA (bovine serum albumin) and 0.25 mM fresh DTT (final concentrations). The reaction was initiated by adding 5 µl [33P]-ATP (1 µCi/sample) mixture containing 0.25mM non-radioactive ATP The blank was determined from a “no substrate” sample.

The reaction was incubated for 15 minutes at 30°C and terminated by spotting 20 μl of the mixture onto phosphocellulose paper strips that were fixed in 1% phosphoric acid and washed three times. Radioactivity was determined using a scintillation counter.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

●

Datasheets/Protocols

40113 Lot 220901

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

● General Protocols

● FAQs

● Contact Technical Service

●

JNK2, GST-Tag Recombinant

PRODUCTS

SERVICES

RESOURCES

COMPANY