AP1 Reporter Kit (JNK Pathway)

Catalog #

60612

$565 *

Size: 500 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The AP1 Reporter Kit is designed for monitoring the activity of the JNK signaling pathway and the transcriptional activity of AP1 in cultured cells. The kit contains a transfection-ready AP1 luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized AP1 responsive elements located upstream of a minimal promoter.

The AP1 reporter is premixed with a constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical for determining pathway-specific effects and the background luciferase activity.

For assays in more difficult to transfect cell types, we suggest using our lentiviral products. The corresponding lentiviral product for this kit is BPS Bioscience #79823.

This product has been cited 9 times.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

JNK reporter, SAPK reporter, Cell based assay kit, cell-based assay, reporter assay kit, activator protein-1, JNK-1, JNK-2

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Product Data Gallery

Product Info

Storage and Usage

Citations9

Supplied As

Reporter: AP1 luciferase reporter vector + constitutively-expressing Renilla luciferase vector
Negative Control Reporter: Non-inducible luciferase vector + constitutively-expressing Renilla luciferase vector

Format

Assay Kit

Concentration

Both the Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each)

Background

The stress-activated protein kinase/c-jun N-terminal kinase (SAPK/JNK) family of proteins includes mitogen-activated protein kinases (MAPKs) that are activated by stress, inflammatory cytokines, mitogens, oncogenes, and inducers of cell differentiation and morphogenesis. Upon activation of the SAPK/JNK pathway, MAP Kinase Kinases phosphorylate and activate JNKs. The activated JNKs translocate to the nucleus where they phosphorylate and activate transcription factors such as c-Jun. The activated c-Jun forms homodimers or heterodimers with fos family proteins which bind to the activator protein-1(AP1) response element and induce target gene transcription.

References

1. Zhou H. et al. (2005) Frequency and distribution of AP-1 sites in the human genome. DNA Research. 11: 139-150.
2. Gaillard P. et.al. (2005) Design and synthesis of the first generation of novel potent, selective, and in vivo active (benzothiazol-2-yl)acetonitrile inhibitors of the c-Jun N-terminal kinase. J Med Chem. 48(14):4596-4607.

Storage/Stability

Stable at least 12 months from date of receipt, when stored as directed (-20°C)

Instructions for Use

See data sheet for detailed transfection and assay protocols.

Applications

• Monitor cAMP/PKA signaling pathway activity.
• Screen for activators or inhibitors of PKA or cAMP/PKA pathway components.
• Study effects of RNAi or gene overexpression on the activity of the cAMP/PKA pathway.

Shipping Temperature

-20°C

Notes

The vectors are suitable for transient transfection. They are NOT suitable for transformation and amplification in bacteria.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

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AP1 Reporter Kit (JNK Pathway)

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