AP1 Luciferase Reporter Lentivirus (JNK Signaling Pathway)

Catalog #

79823

$795 *

Size: 500 µl x 2

Qty

Bulk/Custom

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Description

The stress-activated protein kinase/c-jun N-terminal kinase (SAPK/JNK) family of proteins includes mitogen-activated protein kinases (MAPKs) that are activated by stress, inflammatory cytokines, mitogens, oncogenes, and inducers of cell differentiation and morphogenesis. Upon activation of the SAPK/JNK pathway, MAP Kinase Kinases phosphorylate and activate JNKs. The activated JNKs translocate to the nucleus where they phosphorylate and activate transcription factors such as c-Jun. c-Jun then binds to the activator protein-1 (AP1) response element and induces AP1 transcription.

The AP1 Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by AP1 response element located upstream of the minimal TATA promoter (Figure 1). After transduction, activation of the JNK signaling pathway and AP1 mediated activity in the target cells can be monitored by measuring the luciferase activity.

AP1 Luciferase Reporter Lentivirus

Figure 1. Schematic of the lenti-vector used to generate the ISRE luciferase reporter
lentivirus

This product has been cited 6 times.

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Synonyms

JNK reporter, AP-1 cell line, activator protein-1 lentivirus, activator protein-1, JNK-1, JNK-2

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Product Data Gallery

Product Info

Storage and Usage

Citations6

Supplied As

Two vials (500 μl x 2) of AP1 luciferase reporter lentivirus at a titer 1 x 10⁷TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Materials Required But Not Supplied

• Phorbol 12-Myristate 13-Acetate (PMA) (LC Laboratories, #P-1680). Prepare stock solution in DMSO.
• HEK293 growth medium or use Thaw Medium 9 (BPS Bioscience, #79665): MEM with 10% FBS, 0.1 mM nonessential amino acids, 1 mM sodium pyruvate
• Assay medium: Assay Medium 1B (BPS Bioscience, #79617): Opti-MEM I (Life technologies, #31985062), 0.5% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, and 1% Pen/Strep
• Polybrene (Millipore, #TR-1003-G)
• 96-well tissue culture treated white clear-bottom assay plate (Corning, #3610)
• One-Step luciferase assay system (BPS Bioscience, #60690)
• Luminometer

Formulation

The lentiviruses were produced from HEK293T cells in the medium containing 90% DMEM + 10% FBS.

Storage/Stability

Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Notes

Biosafety

The lentiviruses are produced with the third generation SIN (self-inactivation) lentivector which
ensures self-inactivation of the lentiviral construct after transduction and integration into the
genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the
transduced cells, as they are expressed from packaging plasmids lacking the packing signal.
Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a
Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional
regulations and using all appropriate safety precautions

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

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AP1 Luciferase Reporter Lentivirus (JNK Signaling Pathway)

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