AP-1 Reporter – HEK293 Recombinant Cell Line (JNK signaling pathway)

Catalog #

60405

$2,445 *

Size: 2 vials

Qty

Bulk/Custom

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Description

The stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) family of proteins includes mitogen-activated protein kinases (MAPKs) that are activated by stress, inflammatory cytokines, mitogens, oncogenes, and inducers of cell differentiation and morphogenesis. Upon activation of the SAPK/JNK pathway, MAP Kinase Kinases phosphorylate and activate JNKs. The activated JNKs translocate to the nucleus where they phosphorylate and activate transcription factors such as c-Jun. c-Jun then binds to the activator protein-1 (AP1) response element and induces AP1 transcription.

The AP1 Reporter – HEK293 cell line contains a firefly luciferase gene under the control of AP1-responsive elements that are stably integrated into HEK293 cells. This cell line is validated for its response to stimulation by Phorbol 12-Myristate 13-Acetate (PMA) and to treatment with inhibitors of the JNK signaling pathway.

Interested in screening and profiling inhibitors or activators of SAPK/JNK pathways without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

This product has been cited 2 times.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

JNK reporter, AP-1 cell line, activator protein-1 cell line, activator protein-1, JNK-1, JNK-2

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Product Data Gallery

Dose response of AP1 Reporter – HEK293 cells to PMA

Inhibition of PMA-induced reporter activity by the JNK pathway inhibitor CC-401 in AP1 Reporter – HEK293 cell

Product Info

Storage and Usage

Citations2

Host Cell Line

HEK293 cells

Supplied As

Each vial contains ~1.5 X 10⁶ cells in 1 ml of 10% DMSO.

Materials Required But Not Supplied

Materials Required but Not Supplied for Cell Culture

Materials Required but Not Supplied for Cellular Assay

Phorbol 12-Myristate 13-Acetate (PMA) (Sigma-Aldrich #P-8139). Prepare stock solution in DMSO.
CC-401 (JNK-401) (Sigma-Aldrich #SML-1613): Prepare stock solution in water
Assay Medium 1B (BPS Bioscience #79617)
96-well tissue culture plate or 96-well tissue culture-treated white clear-bottom assay plate
ONE-Step™ Luciferase Assay Reagent (BPS Bioscience #60690)
Luminometer

Biological Activity

This cell line is validated for its response to stimulation by Phorbol 12-Myristate 13-Acetate (PMA) and to treatment with inhibitors of the JNK signaling pathway.

UniProt #

P45983, P45984, P53779

Mycoplasma Testing

The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.

Storage/Stability

Store in liquid nitrogen immediately upon receipt.

Instructions for Use

See data sheet for detailed culturing and assay protocol.

Applications

• Monitor the JNK signaling pathway activity and AP1-mediated activity.
• Screen for activators or inhibitors of the JNK signaling pathway.

Shipping Temperature

-80°C

Notes

License Disclosure

Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide

Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

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Datasheets/Protocols

60405.pdf

SDS

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Certificate of Analysis (CoA)

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AP-1 Reporter – HEK293 Recombinant Cell Line (JNK signaling pathway)

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