ERK2, GST-Tag Recombinant

Catalog #
40299
$410 *
Size: 10 µg
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Description

Recombinant full length human ERK2 (extracellular signal-regulated kinase 2). This construct contains an N-terminal GST-tag. The recombinant protein was affinity purified and was activated by MEK1 in vitro.

Synonyms
MAPK1, P42MAPK, MAPK2, PRKM2, PRKM1, p41mapk, ERT1, p40, p41, ERK, ERK-2
Product Info
Storage and Usage
Citations
Species
Human
Construct
ERK2 (GST-1-360)
Host Species/Expression System
E. coli
Purity

≥80%

Format
Aqueous buffer solution
Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol

MW
68 kDa
Amino Acids
1-360
Specific Activity

24 pmol/min/µg (specific activity measured using the specific activity assay described below)

Genbank #
NM_002745
UniProt #
P28482
Tag(s)
N-terminal GST-tag
Background
ERK2 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK2 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK2 is ubiquitously distributed in tissues with the highest expression in heart, brain and spinal cord. Activated ERK2 translocates into the nucleus where it phosphorylates various transcription factors (e.g., Elk-1, c-Myc, c-Jun, c-Fos, and C/EBP beta).
References
1. Girault, J.A., et al. Curr Opin Pharmacol. Feb,7(1):77-85 (2007)
2. Fyffe-Maricich SL., et al. J. Neurosci. Jan 19,31(3):843-50 (2011).
3. Ossum, C.G., et al. Cell Physiol Biochem. 27(2):109-20 (2011).