Chemi-Verse™ ERK2 Kinase Assay Kit
The Chemi-Verse™ ERK2 Kinase Assay Kit is a luminescence assay designed to measure ERK2 (extracellular signal regulated kinase 2) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant ERK2 kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
- ADP-Glo™ Kinase Assay (Promega #V6930)
- DTT (Dithiothreitol), 1M, optional
- Microplate reader capable of reading luminescence
- Adjustable micropipettor and sterile tips
- 30°C incubator
Catalog # | Name | Amount | Storage |
40299 | ERK2, GST-Tag* | 2.5 µg | -80°C |
79334 | 5x Kinase Buffer 1 | 1.5 ml | -20°C |
79686 | 500 µM ATP | 50 µl | -20°C |
Myelin basic protein (MBP), 5 mg/ml |
50 µl | -20°C | |
79696 | White 96-well plate | 1 | Room Temp |
*The concentration of the protein is lot-specific and will be indicated on the tube.
ERK (extracellular signal-regulated kinases), also known as classical MAPK (mitogen-activated protein kinases), are protein kinases involved in crucial intracellular signaling pathways, and regulate processes like mitoses, meiosis and other functions. In the MAPK/ERK pathway Ras proteins are activated by several external molecules, including cytokines and viruses. These are followed by an activation cascade of c-Raf, MEK (mitogen-activated protein kinase kinase) and finally ERK. ERK2 in turn activates transcription factors. Disruption of the MAPK/ERK pathway at any point results in cancer. Inhibitors for Ras, Raf and MEK have been developed and result in rapid tumor reductions. However, resistance to treatment and relapse are quite frequent. This has led the field to focus on the development of ERK inhibitors, although these are difficult to design due to the high similarity to the CDK (cyclin-dependent kinase) pockets. Further development is needed to make ERK2 inhibitors better tools in cancer therapy.
- Busca R. et al., 2016 Front Cell Dev Biol 4:53.
- Lavoie H., et al., 2020 Nature Reviews Molecular Cell Biology 21:607-623