CDK6/CyclinD3 Kinase Assay Kit
The CDK6/CyclinD3 Kinase Assay Kit is designed to measure CDK6/CyclinD3 kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
- Kinase-Glo MAX (Promega #V6071)
- DTT (Dithiothreitol), 1M, optional
- Microplate reader capable of reading luminescence
- Adjustable micropipettor and sterile tips
- 30°C incubator
| Catalog # | Name | Amount | Storage |
| 40206 | CDK6/CyclinD3* | 20 µg | -80°C |
| 79334 | Kinase assay buffer 1 (5x) | 1.5 ml | -20°C |
| 79686 | ATP (500 µM) | 100 µl | -20°C |
| 78396 | Histone H1 (1 mg/ml) | 500 µl | -20°C |
| 79696 | White 96-well plate | 1 | Room Temp |
*The concentration of the protein is lot-specific and will be indicated on the tube
CDK6 is a serine/threonine protein kinase involved in cell cycle progression and in cellular differentiation. The CDK6/Cyclin D3 complex phosphorylate RB1 to release the transcription factor E2F and drive the transition from G1 to S-phase of the cell cycle. This complex regulates cell proliferation in many cell types and has been validated as a cancer therapeutic target. Palbociclib, a CDK4/CDK6 inhibitor, has shown therapeutic benefit in a small number of patients and is in clinical use for the treatment of ER+/HER2- breast cancer, with more clinical trials ongoing.
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