AP-1 Reporter – HEK293 Recombinant Cell Line (JNK signaling pathway)

Catalog #: 60405
Size: 2 vials
$1,375
Background:
The stress-activated protein kinase/c-jun N-terminal kinase (SAPK/JNK) family of proteins incluces mitogen-activated protein kinases (MAPKs) that are activated by stress, inflammatory cytokines, mitogens, oncogenes, and inducers of cell differentiation and morphogenesis. Upon activation of the SAPK/JNK pathway, MAP Kinase Kinases phosphorylate and activate JNKs. The activated JNKs translocate to the nucleus where they phosphorylate and activate transcription factors such as c-Jun. cJun then binds to the activator protein-1 (AP1) response element and induces AP1 transcription.
Description:
The AP-1 Reporter – HEK293 Cell Line is designed for monitoring the activity of the JNK signaling pathway. The AP1 Reporter – HEK293 cell line contains a firefly luciferase gene under the control of AP1-responsive elements that are stably integrated into HEK293 cells.
Materials Required But Not Provided:

Materials Required but Not Supplied for Cell Culture

Materials Required but Not Supplied for Cellular Assay

  • Phorbol 12-Myristate 13-Acetate (PMA) (LC Laboratories # P-1680). Prepare stock solution in DMSO.
  • JNK inhibitor V (AS601245) (Santa Cruz biotechnology # sc-202672): inhibitor of JNK1,2,3. Prepare stock solution in DMSO.
  • Assay Medium 1B (BPS Cat. #79617)
  • 96-well tissue culture plate or 96-well tissue culture-treated white clear-bottom assay plate
  • ONE-Step™ Luciferase Assay Reagent (BPS Cat. #60690)
  • Luminometer
Synonym(s):
JNK reporter, AP-1 cell line, activator protein-1 cell line
Supplied As:
Each vial contains ~1.5 X 106 cells in 1 ml of 10% DMSO.
Biological Activity:
This cell line is validated for its response to stimulation by Phorbol 12-Myristate 13-Acetate (PMA) and to treatment with inhibitors of the JNK signaling pathway.
Host Cell line:
HEK293 cells
Mycoplasma Testing:
The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Format:
Aqueous solution containing DMSO
Instructions for use:
See data sheet for detailed culturing and assay protocol.
Storage / Stability:
Store in liquid nitrogen immediately upon receipt.
Application(s):
• Monitor the JNK signaling pathway activity and AP1-mediated activity.
• Screen for activators or inhibitors of the JNK signaling pathway.
Reference(s):
1. Zhou H. et al. (2005). 11: 139-150.
2. Gaillard P. et.al. (2005). 48(14):4596-4607.
Notes:

Contact us for pricing.

License Disclosure:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Cell Signaling Pathway
Product Type:
Cell line
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

60405.pdf