CD19 Positive Cell Isolation Kit

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Catalog #

78564

As low as $70 *

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Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The CD19 Positive Cell Isolation Kit is designed to magnetically separate CD19-expressing-cells from a complex immune cell population. This kit is optimized for the isolation of CD19 positive cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, CD19-positive cells will be immobilized along the side of the tube while undesired CD19-negative cells will remain in suspension for easy removal.

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Synonyms

B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12

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Product Data Gallery

Comparison of non-isolated and isolated cell populations

Product Info

Storage and Usage

Citations

Supplied As

This kit provides enough reagents and materials for isolation from up to 1 x 10⁸ or 1 x 10⁹ PBMCs. It is possible to use this kit for multiple isolations from smaller PBMC samples.

Materials Required But Not Supplied

Peripheral blood mononuclear cells (PBMCs) (BPS Bioscience #79059)
Cell Isolation Magnetic Tube Rack (BPS Bioscience #78571)
Centrifuge
15 or 50 ml tubes
Cell counter

Format

1 x 10⁸Cells

Catalog #	Name	Amount	Storage
	Cell Isolation Magnetic Beads	200 µl	+4°C
	CD19 Cell Isolation Antibody	40 µl	-20°C
78563	5x Cell Isolation Buffer	25 ml	+4°C

1 x 10⁹Cells

Catalog #	Name	Amount	Storage
	Cell Isolation Magnetic Beads	2 ml	+4°C
	CD19 Cell Isolation Antibody	400 µl	-20°C
78563	5x Cell Isolation Buffer	250 ml	+4°C

UniProt #

P15391

Background

B cells are lymphocytes that play a critical role in the humoral immune response. These cells produce antibodies, present antigens, and secrete cytokines. CD19 is a canonical marker of B cells and is present at most stages of B cell development. In PBMCs derived from healthy individuals, about 5-10% of the cells are CD19⁺ B cells.

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CD19 Positive Cell Isolation Kit

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