Anti-CD19/CD22 Bispecific CAR Lentivirus (Clones FMC63/m971 ScFv-CD8-4-1BB-CD3ζ)

Catalog #

78609

$1,095 *

Size: 50 µl

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The anti-CD19/CD22 Bispecific CAR lentiviruses are replication incompetent, HIV-based, VSV-G-pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. These viruses transduce the ScFv (single-chain variable fragments) of anti-CD19 (clone FMC63) and anti-CD22 (clone m971) linked to a 2^nd generation CAR (Chimeric Antigen Receptor) containing CD8 hinge and transmembrane domains, and the 4-1BB and CD3ζ signaling domains (Figure 1).

Figure 1. (A) Schematic of the lenti-vector used to generate the anti-CD19/CD22 Bispecific CAR lentivirus and (B) Construct diagram showing components of the anti-CD19/CD22 CAR.

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Product Info

Storage and Usage

Citations

Supplied As

50 µl of anti-CD19/CD22 Bispecific CAR Lentivirus at a titer ≥ 10⁸ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Materials Required But Not Supplied

Name	Ordering Information
PBMC, Frozen	BPS Bioscience #79059
Human Interleukin-2	BPS Bioscience #90184
EasySep™ Human CD4+ T Cell Isolation Kit	Stemcell technologies #17952
EasySep™ Human CD8+ T Cell Isolation Kit	Stemcell technologies #17953
Human CD3/CD28/CD2 T Cell Activator	Stemcell technologies #10970
PE-Labeled Anti-FMC63 scFv Monoclonal Antibody	Acrobiosystems, # FM3-HPY53-25tests
Biotinylated Human CD22 Protein	Acrobiosystems #SI2-H82F8-25ug
PE-Streptavidin	Biolegend #405203
CD19/Firefly Luciferase CHO Cell Line	BPS Bioscience #79714
CD22/Firefly Luciferase CHO Cell Line	BPS Bioscience #79715
Firefly Luciferase CHO Cell Line	BPS Bioscience #79725
Firefly Luciferase Raji Cell Line	BPS Bioscience #78622
Firefly Luciferase K562 Cell Line	BPS Bioscience #78621
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690

Formulation

The lentiviruses were produced from HEK293T cells, concentrated, and resuspended in DMEM.

Storage/Stability

Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.

Applications

Ideal as a positive control for anti-CD19/CD22 Bispecific CAR evaluation in T cells
Use in transduction optimization
Use to generate anti-CD19/CD22 Bispecific CAR-T cells for research use only, not for therapeutic purposes

Shipping Temperature

-80°C

Notes

Biosafety
The lentiviruses are produced with the SIN (self-inactivation) lentivector which ensures self-inactivation of the lentiviral construct after transduction and integration into the genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal and are not present in the lentivirus particle. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS Bioscience recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.

Troubleshooting Guide
Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

CAR-T

Regulatory Status

Research Use Only

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Anti-CD19/CD22 Bispecific CAR Lentivirus (Clones FMC63/m971 ScFv-CD8-4-1BB-CD3ζ)

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