PBMC Cytotoxicity Luciferase Assay Kit (NALM6)
The PBMC Cytotoxicity Luciferase Assay Kit (NALM6) is a kit designed to determine the cytotoxicity profile of PBMCs (Peripheral Blood Mononuclear Cells) towards the Firefly Luciferase NALM6 Cell Line. It uses the luminescence signal from Firefly Luciferase NALM6 Cell Line to measure the number of live target cells within a mixed cell population of PBMC and Firefly Luciferase NALM6 cells. The kit contains PBMCs, Firefly Luciferase NALM6 Cell Line, cell culture media and One-Step™ Luciferase Assay System.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
- 96 Well White, Clear Bottom Plate
- T75 cell culture flask.
- Luminometer.
| Catalog # | Name | Amount | Storage |
| 79059 | Normal Human Peripheral Blood Mononuclear Cells, Frozen | 2 vials at 10 x 106 cells each | Liquid Nitrogen |
| 78494 | Firefly Luciferase NALM6 Cell Line | 2 vials at ˃1 million cells each | Liquid Nitrogen |
| 60184 | Thaw Medium 2 | 2 x 100 ml | 4°C |
| 79639 | Growth Medium 2D | 1 x 100 ml | 4°C |
| 60690-1 | ONE-Step™ Luciferase Assay System | 2 x 10 ml kit | -20°C |
Lymphocyte-mediated cytotoxicity is a form of cellular immunity against intracellular pathogens, including viruses and certain bacteria and parasites. The most popular in vitro methods to monitor lymphocyte-mediated cytotoxicity on target cells are cell-mediated cytotoxicity assays such as ADCC (antibody-dependent cellular cytotoxicity) and TDCC (T-cell dependent cellular cytotoxicity) in which immune effector cells and target cells are co-cultured. To analyze immune effector cell cytolytic activity in such heterogeneous cell population of effector and target cells, it is important to be able to discriminate between effector and target cell populations with distinct phenotypes. The use of luciferase allows for a clear separation between the effector and the surviving target cells. The instability of firefly luciferase when released from dead target cells in cell culture gives it a half-life of approximately 2 hours, eliminating any residual luminescence signal generated from dead target cells. Cytotoxicity assays are crucial to understand the potency of CAR (chimeric antigen receptor) T and NK cells, and antibody-based immunotherapies.
NALM6 is a human B cell precursor leukemia cell line derived from the peripheral blood of a patient with acute lymphoblastic leukemia. The Firefly Luciferase NALM6 Cell Line constitutively expresses the Firefly (Photinus pyralis) luciferase reporter under the control of a CMV promoter. Luciferase activity is directly proportional to live cell numbers.
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