Anti-CD4 Antibody, PE-Labeled

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Catalog #

102010

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Description

This anti-CD4 antibody is a purified recombinant antibody, which is labeled with R-Phycoerythrin (PE). This antibody has been tested by flow cytometry for specific binding to human CD4 on the surface of PBMCs (BPS Bioscience #79059).

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Product Data Gallery

Cell surface expression of CD4 in PBMCs.

Product Info

Storage and Usage

Citations

Species

Human

Isotype

IgG1

Clonality

Monoclonal

Host Species/Expression System

HEK293

Purity

≥90%

Purification

Protein A affinity chromatography from HEK293 supernatants.

Formulation

8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain a protein stabilizer.

Label

PE is an oligomeric protein complex (270 kDa) from red algae that exhibits intensely bright red-orange fluorescence with excitation peak at 566 nm and an emission peak at 574 nm. The complex consists of six heterodimers, α subunit (18 kDa) and β-subunit (20 kDa), and an additional γ-subunit (34 kDa).

Background

CD4 (cluster of differentiation 4) is part of the immunoglobulin superfamily, and is found in T-helper cells, monocytes, macrophages and dendritic cells. It is a co-receptor in the TCR (T-cell receptor), binding to MHC (major histocompatibility complex) class II molecules. CD4 binds to the tyrosine kinase Lck (lymphocyte-specific protein tyrosine kinase), which can phosphorylate the ITAM (immunoreceptor tyrosine activation motifs) domain of the CD3, activating CD3-related signaling. CD4 is a typical T cell marker of T helper cells, and it has been linked to cancer, autoimmune diseases such as vitiligo and type I diabetes. In addition, HIV-1 makes use of CD4 to trigger viral envelope protein conformational changes that allow cell infection. Ibalizumab, an anti-CD4 antibody, is currently used in the treatment of HIV, being considered a first-in-class medication. Further studies into CD4 and potential strategies around it may benefit patients and CD4 related diseases.

Storage/Stability

At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.

Assay Conditions

One million PBMCs (BPS Bioscience #79059) were blocked and stained with 1 µg of anti-CD4 antibody, PE-labeled (BPS Bioscience #102010) or PE Human IgG1 Isotype Control (BioLegend #403503) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. Non-lymphoid cells were excluded from the analysis based on side scatter and forward scatter signals. Y-axis represents the cell count while X-axis indicates the PE intensity.

Instructions for Use

For flow cytometry staining, the suggested use of this reagent is 5 µl (1 µg antibody) per million cells in 100 µl staining volume.

Applications

Useful for studying expression of CD4 on the cell surface by flow cytometry.

Shipping Temperature

+4°C (gel packs)

Cross Reactivity

This antibody recognizes human CD4. It has not been tested on other species.

Warnings

Do not freeze. Protect from light.

Scientific Category

Immune Cell Markers

Regulatory Status

Research Use Only

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Anti-CD4 Antibody, PE-Labeled

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