Anti-NCAM1 Antibody, FITC-Labeled

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Catalog #

101865

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Description

This anti-NCAM1 antibody is a purified recombinant antibody, which is labeled with fluorescein isothiocyanate (FITC). This antibody has been tested by flow cytometry for specific binding to human NCAM1 on the surface of NCAM1 CHO Recombinant Cell Line (BPS Bioscience #78352).

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Synonyms

Neural cell adhesion molecule 1, N-CAM-1, NCAM-1, CD56, NCAM1, NCAM

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Product Data Gallery

Cell surface expression of NCAM1 in NCAM1 CHO Cell Line.

Product Info

Storage and Usage

Citations

Species

Human

Isotype

IgG1

Clonality

Monoclonal

Host Species/Expression System

HEK293

Purity

≥90%

Purification

Protein A affinity chromatography from HEK293 supernatant.

Format

Aqueous buffer solution.

Formulation

8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain a protein stabilizer.

UniProt #

P13591

Label

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein and has excitation and emission spectrum peak wavelengths of approximately 495 nm and 519 nm, causing it to fluoresce green.

Background

NCAM1 (Neural cell adhesion molecule 1), also known as CD56 (Cluster of Differentiation 56), is a cell surface glycoprotein expressed typically in NK (Natural Killer) cells. Its level correlates with the cytotoxicity potential of the NK cells. Deficiencies in CD56⁺ immune cells have been associated with specific infections and autoimmune diseases. NCAM1 expression is altered in hematological malignancies, such as multiple myeloma and leukemia, and solid tumor cells from various tissue types. Studies of NCAM1 function and expression patterns will continue to broaden our knowledge and support possible applications in cancer therapy.

Storage/Stability

At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.

Assay Conditions

One million NCAM1 CHO cells (BPS Bioscience #78352) were blocked and stained with 1 µg of anti-NCAM1 antibody, FITC-labeled (BPS Bioscience #101865) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. NCAM1 CHO cells (green) were compared to parental CHO-K1 cells (blue). Y-axis represents the cell count, while X-axis shows FITC intensity.

Applications

Useful for studying expression of NCAM1 on the cell surface by flow cytometry. For flow cytometric staining, the suggested use of this reagent is 5 µl (1 µg antibody) per million cells in 100 µl staining volume.

Shipping Temperature

+4°C (gel packs)

Cross Reactivity

This antibody recognizes human NCAM1. It has not been tested in other species.

Warnings

Do not freeze. Protect from light.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Anti-NCAM1 Antibody, FITC-Labeled

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