Anti-CD3 Antibody, FITC-Labeled

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Catalog #

102008

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Description

This anti-CD3 antibody is a purified recombinant antibody, which is labeled with fluorescein isothiocyanate (FITC). This antibody has been tested by flow cytometry for specific binding to human CD3 on the surface of PBMCs (BPS Bioscience #79059).

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Product Data Gallery

Cell surface expression of CD3 in PBMCs.

Product Info

Storage and Usage

Citations

Species

Human

Isotype

IgG1

Clonality

Monoclonal

Host Species/Expression System

HEK293

Purity

≥90%

Purification

Protein A affinity chromatography from HEK293 supernatant.

Format

Aqueous buffer solution.

Label

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein and has excitation and emission spectrum peak wavelengths of approximately 495 nm and 519 nm, causing it to fluoresce green.

Background

CD3 (cluster of differentiation 3) is a complex made of CD3γ chain, a CD3δ chain and two CD3ε chains. CD3 associates with the TCR (T-cell receptor) and a CD3ζ chain to activate cytotoxic T Cells (CD8⁺) and T helper cells (CD4⁺) and form the TCR complex. It is found in T cells, during all developmental stages, and it is a typical T cell marker. Its essential role in T cell activation has made CD3 an attractive and useful molecule for the development of anti-cancer drugs. For example, CAR-T cells (chimeric receptor antigen T cells) made use of the CD3ζ domain for intracellular signaling activation.

Storage/Stability

At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE.

Assay Conditions

One million PBMCs (BPS Bioscience #79059) were blocked and stained with 1 µg of anti-CD3 antibody, FITC-labeled (BPS Bioscience #102008) or FITC Human IgG1 Isotype Control (BioLegend #403507) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. Non-lymphoid cells were excluded from the analysis based on side scatter and forward scatter signals. Y-axis represents the cell count, while X-axis shows FITC intensity.

Applications

Useful for studying expression of CD3 on the cell surface by flow cytometry. For flow cytometric staining, the suggested use of this reagent is 5 µl (1 µg antibody) per million cells in 100 µl staining volume.

Shipping Temperature

+4°C (gel packs)

Cross Reactivity

This antibody recognizes human CD3. It has not been tested on other species.

Warnings

Do not freeze. Protect from light.

Regulatory Status

Research Use Only

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Anti-CD3 Antibody, FITC-Labeled

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