Anti-CD3 Antibody, PE-Labeled

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Catalog #

102009

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Description

This anti-CD3 antibody is a purified recombinant antibody, which is labeled with R-Phycoerythrin (PE). This antibody has been tested by flow cytometry for specific binding to human CD3 on the surface of PBMCs (BPS Bioscience #79059).

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Product Data Gallery

Cell surface expression of CD3 in PBMCs.

Product Info

Storage and Usage

Citations

Species

Human

Clonality

Monoclonal

Host Species/Expression System

HEK293

Purity

≥90%

Purification

Protein A affinity chromatography from HEK293 supernatants.

Formulation

8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain a protein stabilizer.

Label

PE is an oligomeric protein complex (270 kDa) from red algae that exhibits intensely bright red-orange fluorescence with excitation peak at 566 nm and an emission peak at 574 nm. The complex consists of six heterodimers, α subunit (18 kDa) and β-subunit (20 kDa), and an additional γ-subunit (34 kDa).

Background

CD3 (cluster of differentiation 3) is a complex made of CD3γ chain, a CD3δ chain and two CD3ε chains. CD3 associates with the TCR (T-cell receptor) and a CD3ζ chain to activate cytotoxic T Cells (CD8⁺) and T helper cells (CD4⁺) and form the TCR complex. It is found in T cells, during all developmental stages, and it is a typical T cell marker. Its essential role in T cell activation has made CD3 an attractive and useful molecule for the development of anti-cancer drugs. For example, CAR-T cells (chimeric receptor antigen T cells) made use of the CD3ζ domain for intracellular signaling activation.

Storage/Stability

At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.

Assay Conditions

One million PBMCs (BPS Bioscience #79059) were blocked and stained with 1 µg of anti-CD3 antibody, PE-Labeled (BPS Bioscience #102009) or PE Human IgG1 Isotype Control (BioLegend #403503) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. Non-lymphoid cells were excluded from the analysis based on side scatter and forward scatter signals. Y-axis represents the cell count while the X-axis indicates PE intensity.

Applications

Useful for studying expression of CD3 on the cell surface by flow cytometry.

Shipping Temperature

+4°C (gel packs)

Cross Reactivity

This antibody recognizes human CD3. It has not been tested on other species.

Warnings

Do not freeze. Protect from light.

Scientific Category

Immune Cell Markers

Regulatory Status

Research Use Only